BMRB Entry 27643

Title:
Chemical shifts of the WH domain of human ORC2
Deposition date:
2018-10-09
Original release date:
2018-11-08
Authors:
Zhang, Jiahai
Citation:

Citation: Zhang, Jiahai; Guo, Qiong; Gao, Jun; Xu, Chao; Zhang, Beibei; Li, Mingwei; She, Jiaqi; Shi, Yunyu; Zhang, Zhiyong; Ruan, Ke; Wu, Jihui. "Structural insight into the unique dsDNA binding topology of the human ORC2 wing helix domain"  FEBS J. 286, 2726-2736 (2019).
PubMed: 30963726

Assembly members:

Assembly members:
WH_Domain_of_Human_ORC2, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: peT28-MHL

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts434
15N chemical shifts114
1H chemical shifts715

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WH DOmain of ORC21

Entities:

Entity 1, WH DOmain of ORC2 107 residues - Formula weight is not available

1   SERLEUPROLEUSERSERLEUTHRHISVAL
2   LEUARGSERLEUTHRPROASNALAARGGLY
3   ILEPHEARGLEULEUILELYSTYRGLNLEU
4   ASPASNGLNASPASNPROSERTYRILEGLY
5   LEUSERPHEGLNASPPHETYRGLNGLNCYS
6   ARGGLUALAPHELEUVALASNSERASPLEU
7   THRLEUARGALAGLNLEUTHRGLUPHEARG
8   ASPHISLYSLEUILEARGTHRLYSLYSGLY
9   THRASPGLYVALGLUTYRLEULEUILEPRO
10   VALASPASNGLYTHRLEUTHRASPPHELEU
11   GLULYSGLUGLUGLUGLUALA

Samples:

sample_1: WH_Domain_of_Human_ORC2, [U-99% 13C; U-99% 15N], 0.8 mM; phosphate sodium 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks