BMRB Entry 27633

Title:
Chemical shift assignments of the C-terminal domain of chicken H1.11L
Deposition date:
2018-09-27
Original release date:
2019-02-06
Authors:
Turner, Abigail; Watson, Matthew; Wilkins, Oscar; Cato, Laura; Travers, Andrew; Thomas, Jean; Stott, Katherine
Citation:

Citation: Turner, Abigail; Watson, Matthew; Wilkins, Oscar; Cato, Laura; Travers, Andrew; Thomas, Jean; Stott, Katherine. "Highly disordered histone H1-DNA model complexes and their condensates"  Proc. Natl. Acad. Sci. U.S.A. 115, 11964-11969 (2018).
PubMed: 30301810

Assembly members:

Assembly members:
H1, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET13a

Data sets:
Data typeCount
15N chemical shifts71
1H chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H1 C-terminal domain1

Entities:

Entity 1, H1 C-terminal domain 111 residues - Formula weight is not available

1   LYSPROGLYGLUVALLYSGLULYSALAPRO
2   LYSLYSLYSALASERALAALALYSPROLYS
3   LYSPROALAALALYSLYSPROALAALAALA
4   ALALYSLYSPROLYSLYSALAVALALAVAL
5   LYSLYSSERPROLYSLYSALALYSLYSPRO
6   ALAALASERALATHRLYSLYSSERALALYS
7   SERPROLYSLYSVALTHRLYSALAVALLYS
8   PROLYSLYSALAVALALAALALYSSERPRO
9   ALALYSALALYSALAVALLYSPROLYSALA
10   ALALYSPROLYSALAALALYSPROLYSALA
11   ALALYSALALYSLYSALAALAALALYSLYS
12   LYS

Samples:

sample_1: H1, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 0.5 mM; NaCl 0.5 mM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

AZARA, Boucher - processing

Analysis vv2.4, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks