BMRB Entry 27628

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for SIRT1 183-233
Published: 2018-11-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27628

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for SIRT1 183-233

Deposition date:

2018-09-26

Original release date:

2018-11-02

Authors:

Krzysiak, Troy; Gronenborn, Angela

Citation:

Citation: Krzysiak, Troy; Thomas, Laurel; Choi, You-Jin; Auclair, Sylvain; Qian, Yiqi; Luan, Shan; Krasnow, Stephanie; Thomas, Laura; Koharudin, Leonardus; Benos, Panayiotis; Marks, Daniel; Gronenborn, Angela; Thomas, Gary. "An Insulin-Responsive Sensor in the SIRT1 Disordered Region Binds DBC1 and PACS-2 to Control Enzyme Activity." Mol. Cell 72, 985-998 (2018).
PubMed: 30415949

Assembly members:

Assembly members:
SIRT1_183-233, polymer, 54 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41 TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SIRT1_183-233: SEFGPYTFVQQHLMIGTDPR TILKDLLPETIPPPELDDMT LWQIVINILSEPPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	109
15N chemical shifts	45
1H chemical shifts	45

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SIRT1_183-233	1

Entities:

Entity 1, SIRT1_183-233 54 residues - Formula weight is not available

1

SER

GLU

PHE

GLY

PRO

TYR

THR

PHE

VAL

GLN

2

GLN

HIS

LEU

MET

ILE

GLY

THR

ASP

PRO

ARG

3

THR

ILE

LEU

LYS

ASP

LEU

PRO

GLU

THR

4

ILE

PRO

GLU

LEU

ASP

MET

THR

5

LEU

TRP

GLN

ILE

VAL

ILE

ASN

ILE

LEU

SER

6

GLU

PRO

LYS

Samples:

sample_1: SIRT1_183-233, [U-13C; U-15N], 100 uM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks