BMRB Entry 27626

Name: 1H, 13C, and 15N Chemical Shift Assignments for the SAH domain from mouse myosin 7a
Published: 2019-01-02

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27626

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for the SAH domain from mouse myosin 7a

Deposition date:

2018-09-25

Original release date:

2019-01-02

Authors:

Batchelor, Matthew; Peckham, Michelle

Citation:

Citation: Batchelor, Matthew; Wolny, Marcin; Baker, Emily; Paci, Emanuele; Kalverda, Arnout; Peckham, Michelle. "Dynamic ion pair behavior stabilizes single alpha-helices in proteins" J. Biol. Chem. 294, 3219-3234 (2019).
PubMed: 30593502

Assembly members:

Assembly members:
M7A_SAH, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M7A_SAH: SRLRVEYQRRLEAERMRLAE EEKLRKEMSAKKAKEEAERK HQERLAQLAREDAERELKEK EEARRKKELLEQMEKARHEW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	391
15N chemical shifts	83
1H chemical shifts	552

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	M7A SAH, 1	1
2	M7A SAH, 2	1

Entities:

Entity 1, M7A SAH, 1 80 residues - Formula weight is not available

1	SER	ARG	LEU	ARG	VAL	GLU	TYR	GLN	ARG	ARG
2	LEU	GLU	ALA	GLU	ARG	MET	ARG	LEU	ALA	GLU
3	GLU	GLU	LYS	LEU	ARG	LYS	GLU	MET	SER	ALA
4	LYS	LYS	ALA	LYS	GLU	GLU	ALA	GLU	ARG	LYS
5	HIS	GLN	GLU	ARG	LEU	ALA	GLN	LEU	ALA	ARG
6	GLU	ASP	ALA	GLU	ARG	GLU	LEU	LYS	GLU	LYS
7	GLU	GLU	ALA	ARG	ARG	LYS	LYS	GLU	LEU	LEU
8	GLU	GLN	MET	GLU	LYS	ALA	ARG	HIS	GLU	TRP

Samples:

sample_1: M7A SAH, [U-100% 13C; U-100% 15N], 0.4 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 23.4 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(C)CH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D (HC)CO(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Ascend AEON 950 MHz
Bruker Avance 750 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	SER	ARG	LEU	ARG	VAL	GLU	TYR	GLN	ARG	ARG
2	LEU	GLU	ALA	GLU	ARG	MET	ARG	LEU	ALA	GLU
3	GLU	GLU	LYS	LEU	ARG	LYS	GLU	MET	SER	ALA
4	LYS	LYS	ALA	LYS	GLU	GLU	ALA	GLU	ARG	LYS
5	HIS	GLN	GLU	ARG	LEU	ALA	GLN	LEU	ALA	ARG
6	GLU	ASP	ALA	GLU	ARG	GLU	LEU	LYS	GLU	LYS
7	GLU	GLU	ALA	ARG	ARG	LYS	LYS	GLU	LEU	LEU
8	GLU	GLN	MET	GLU	LYS	ALA	ARG	HIS	GLU	TRP

1	SER	ARG	LEU	ARG	VAL	GLU	TYR	GLN	ARG	ARG
2	LEU	GLU	ALA	GLU	ARG	MET	ARG	LEU	ALA	GLU
3	GLU	GLU	LYS	LEU	ARG	LYS	GLU	MET	SER	ALA
4	LYS	LYS	ALA	LYS	GLU	GLU	ALA	GLU	ARG	LYS
5	HIS	GLN	GLU	ARG	LEU	ALA	GLN	LEU	ALA	ARG
6	GLU	ASP	ALA	GLU	ARG	GLU	LEU	LYS	GLU	LYS
7	GLU	GLU	ALA	ARG	ARG	LYS	LYS	GLU	LEU	LEU
8	GLU	GLN	MET	GLU	LYS	ALA	ARG	HIS	GLU	TRP

1	SER	ARG	LEU	ARG	VAL	GLU	TYR	GLN	ARG	ARG
2	LEU	GLU	ALA	GLU	ARG	MET	ARG	LEU	ALA	GLU
3	GLU	GLU	LYS	LEU	ARG	LYS	GLU	MET	SER	ALA
4	LYS	LYS	ALA	LYS	GLU	GLU	ALA	GLU	ARG	LYS
5	HIS	GLN	GLU	ARG	LEU	ALA	GLN	LEU	ALA	ARG
6	GLU	ASP	ALA	GLU	ARG	GLU	LEU	LYS	GLU	LYS
7	GLU	GLU	ALA	ARG	ARG	LYS	LYS	GLU	LEU	LEU
8	GLU	GLN	MET	GLU	LYS	ALA	ARG	HIS	GLU	TRP