BMRB Entry 27622

Name: Backbone assignment of HOX protein SCR
Published: 2019-03-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27622

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of HOX protein SCR

Deposition date:

2018-09-24

Original release date:

2019-03-28

Authors:

Maiti, Snigdha; Acharya, Bidisha; De, Soumya

Citation:

Citation: Maiti, Snigdha; Acharya, Bidisha; Boorla, Veda Sheersh; Manna, Bharat; Ghosh, Amit; De, Soumya. "Dynamic Studies on Intrinsically Disordered Regions of Two Paralogous Transcription Factors Reveal Rigid Segments with Important Biological Functions" J. Mol. Biol. 431, 1353-1369 (2019).
PubMed: 30802457

Assembly members:

Assembly members:
SCR, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28 (a+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SCR: KKNPPQIYPWMKRVHLGTST VNANGETKRQRTSYTRYQTL ELEKEFHFNRYLTRRRRIEI AHALSLTERQIKIWFQNRRM KWKKEHK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	85
1H chemical shifts	160

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SCR monomer	1

Entities:

Entity 1, SCR monomer 87 residues - Formula weight is not available

1

LYS

ASN

PRO

GLN

ILE

TYR

PRO

TRP

2

MET

LYS

ARG

VAL

HIS

LEU

GLY

THR

SER

THR

3

VAL

ASN

ALA

ASN

GLY

GLU

THR

LYS

ARG

GLN

4

ARG

THR

SER

TYR

THR

ARG

TYR

GLN

THR

LEU

5

GLU

LEU

GLU

LYS

GLU

PHE

HIS

PHE

ASN

ARG

6

TYR

LEU

THR

ARG

ILE

GLU

ILE

7

ALA

HIS

ALA

LEU

SER

LEU

THR

GLU

ARG

GLN

8

ILE

LYS

ILE

TRP

PHE

GLN

ASN

ARG

MET

9

LYS

TRP

LYS

GLU

HIS

LYS

Samples:

sample_1: SCR, [U-100% 13C; U-100% 15N], 0.36 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks