BMRB Entry 27621

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27621

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Title:
Backbone 1H, 15N, 13C assignment of DfdT337-K426.
Deposition date:
2018-09-24
Original release date:
2019-03-28
Authors:
Acharya, Bidisha; Maiti, Snigdha; De, Soumya
Citation:

Citation: Maiti, Snigdha; Acharya, Bidisha; Boorla, Veda Sheersh; Manna, Bharat; Ghosh, Amit; De, Soumya. "Dynamic Studies on Intrinsically Disordered Regions of Two Paralogous Transcription Factors Reveal Rigid Segments with Important Biological Functions"  J. Mol. Biol. 431, 1353-1369 (2019).
PubMed: 30802457

Assembly members:

Assembly members:
DFD, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DFD: TDGERIIYPWMKKIHVAGVA NGSYQPGMEPKRQRTAYTRH QILELEKEFHYNRYLTRRRR IEIAHTLVLSERQIKIWFQN RRMKWKKDNK

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts89
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DFD monomer1

Entities:

Entity 1, DFD monomer 90 residues - Formula weight is not available

1   THRASPGLYGLUARGILEILETYRPROTRP
2   METLYSLYSILEHISVALALAGLYVALALA
3   ASNGLYSERTYRGLNPROGLYMETGLUPRO
4   LYSARGGLNARGTHRALATYRTHRARGHIS
5   GLNILELEUGLULEUGLULYSGLUPHEHIS
6   TYRASNARGTYRLEUTHRARGARGARGARG
7   ILEGLUILEALAHISTHRLEUVALLEUSER
8   GLUARGGLNILELYSILETRPPHEGLNASN
9   ARGARGMETLYSTRPLYSLYSASPASNLYS

Samples:

sample_1: DFD, [U-100% 13C; U-100% 15N], 0.3 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY v3.131, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks