BMRB Entry 27616

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for CBX8 chromodomain
Deposition date:
2018-09-19
Original release date:
2018-12-17
Authors:
Weaver, Tyler; Musselman, Catherine
Citation:

Citation: Connelly, Katelyn; Weaver, Tyler; Alpsoy, Aktan; Gu, Brian; Musselman, Catherine; Dykhuizen, Emily. "Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association."  Nucleic Acids Res. 47, 2289-2305 (2019).
PubMed: 30597065

Assembly members:

Assembly members:
CBX8_CD, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGSTag

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts101
15N chemical shifts49
1H chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBX8 CD1

Entities:

Entity 1, CBX8 CD 55 residues - Formula weight is not available

1   GLYGLUARGVALPHEALAALAGLUALALEU
2   LEULYSARGARGILEARGLYSGLYARGMET
3   GLUTYRLEUVALLYSTRPLYSGLYTRPSER
4   GLNLYSTYRSERTHRTRPGLUPROGLUGLU
5   ASNILELEUASPALAARGLEULEUALAALA
6   PHEGLUGLUARGGLU

Samples:

sample_1: CBX8 CD, [U-13C; U-15N], 275 uM; sodium chloride 100 mM; sodium phosphate 40 mM

sample_conditions_1: ionic strength: 0.14 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks