BMRB Entry 27601

Title:
RXDP2ext(1-73)
Deposition date:
2018-09-09
Original release date:
2018-10-09
Authors:
Sethi, Ashish; Gooley, Paul
Citation:

Citation: Hoare, Bradley; Bruell, Shoni; Sethi, Ashish; Gooley, Paul; Lew, Michael; Hossain, Mohammed; Inoue, Asuka; Scott, Daniel; Bathgate, Ross. "Multi-Component Mechanism of H2 Relaxin Binding to RXFP1 through NanoBRET Kinetic Analysis."  iScience 11, 93-9113 (2019).
PubMed: 30594862

Assembly members:

Assembly members:
RXFP2ext, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RXFP2ext1

Entities:

Entity 1, RXFP2ext 128 residues - Formula weight is not available

1   METILETHRPHESERCYSGLNLYSGLYTYR
2   PHEPROCYSGLYASNLEUTHRLYSCYSLEU
3   PROARGALAPHEHISCYSASPGLYLYSASP
4   ASPCYSGLYASNGLYALAASPGLUGLUASN
5   CYSGLYASPTHRSERGLYTRPALATHRILE
6   PHEGLYLYSTYRPHEALASERTYRTYRTHR
7   VALHISGLYASNALAASNSERVALALALEU
8   THRGLNGLUGLNTYRLYSLEUALALEUASN
9   GLYLYSTHRLEULYSGLYGLUTHRTHRTHR
10   GLUALAVALASPALAALATHRALAGLULYS
11   VALPHELYSGLNTYRALAASNASPASNGLY
12   VALASPGLYGLUTRPTHRTYRASPASPALA
13   THRLYSTHRPHETHRVALTHRGLU

Samples:

sample_1: RXFP2ext, [U-100% 13C; U-100% 15N], 65 uM; imidazole 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks