BMRB Entry 27576

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for A97S TTR in 5% DMSO
Deposition date:
2018-08-13
Original release date:
2018-09-12
Authors:
Yu, Tsyr-Yan; Yen, Yueh-Jung; Chang, Yu; Huang, Shing-Jong
Citation:

Citation: Liu, Yo-Tsen; Yen, Yueh-Jung; Ricardo, Frans; Chang, Yu; Wu, Pei-Hao; Huang, Shing-Jong; Lin, Kon-Ping; Yu, Tsyr-Yan. "Biophysical characterization and modulation of Transthyretin Ala97Ser"  Ann. Clin. Transl. Neurol. 6, 1961-1970 (2019).
PubMed: 31502419

Assembly members:

Assembly members:
A97S_TTR, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Data sets:
Data typeCount
13C chemical shifts126
15N chemical shifts117
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A97S TTR1

Entities:

Entity 1, A97S TTR 127 residues - Formula weight is not available

A97S mutation

1   GLYPROTHRGLYTHRGLYGLUSERLYSCYS
2   PROLEUMETVALLYSVALLEUASPALAVAL
3   ARGGLYSERPROALAILEASNVALALAVAL
4   HISVALPHEARGLYSALAALAASPASPTHR
5   TRPGLUPROPHEALASERGLYLYSTHRSER
6   GLUSERGLYGLULEUHISGLYLEUTHRTHR
7   GLUGLUGLUPHEVALGLUGLYILETYRLYS
8   VALGLUILEASPTHRLYSSERTYRTRPLYS
9   ALALEUGLYILESERPROPHEHISGLUHIS
10   ALAGLUVALVALPHETHRSERASNASPSER
11   GLYPROARGARGTYRTHRILEALAALALEU
12   LEUSERPROTYRSERTYRSERTHRTHRALA
13   VALVALTHRASNPROLYSGLU

Samples:

sample_1: A97S TTR, [U-13C; U-15N; U-2H], 0.75 mM; D2O, [U-99% 2H], 6%; sodium phosphate 25 mM; sodium chloride 100 mM; EDTA 0.5 mM; DMSO 5%; TCEP 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 37 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA, Rochus Keller - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks