BMRB Entry 27558

Title:
Amide chemical shifts of PCNA bound to p15 peptide and DNA
Deposition date:
2018-07-25
Original release date:
2018-11-15
Authors:
De Biasio, Alfredo; Blanco, Francisco
Citation:

Citation: De March, Matteo; Barrera-Vilarmau, Susana; Crespan, Emmanuele; Mentegari, Elisa; Merino, Nekane; Gonzalez-Magana, Amaia; Romano-Moreno, Miguel; Maga, Giovanni; Crehuet, Ramon; Onesti, Silvia; Blanco, Francisco; De Biasio, Alfredo. "p15PAF binding to PCNA modulates the DNA sliding surface."  Nucleic Acids Res. 46, 9816-9828 (2018).
PubMed: 30102405

Assembly members:

Assembly members:
PCNA, polymer, 264 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
15N chemical shifts151
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PCNA1

Entities:

Entity 1, PCNA 264 residues - Formula weight is not available

1   GLYPROHISMETPHEGLUALAARGLEUVAL
2   GLNGLYSERILELEULYSLYSVALLEUGLU
3   ALALEULYSASPLEUILEASNGLUALACYS
4   TRPASPILESERSERSERGLYVALASNLEU
5   GLNSERMETASPSERSERHISVALSERLEU
6   VALGLNLEUTHRLEUARGSERGLUGLYPHE
7   ASPTHRTYRARGCYSASPARGASNLEUALA
8   METGLYVALASNLEUTHRSERMETSERLYS
9   ILELEULYSCYSALAGLYASNGLUASPILE
10   ILETHRLEUARGALAGLUASPASNALAASP
11   THRLEUALALEUVALPHEGLUALAPROASN
12   GLNGLULYSVALSERASPTYRGLUMETLYS
13   LEUMETASPLEUASPVALGLUGLNLEUGLY
14   ILEPROGLUGLNGLUTYRSERCYSVALVAL
15   LYSMETPROSERGLYGLUPHEALAARGILE
16   CYSARGASPLEUSERHISILEGLYASPALA
17   VALVALILESERCYSALALYSASPGLYVAL
18   LYSPHESERALASERGLYGLULEUGLYASN
19   GLYASNILELYSLEUSERGLNTHRSERASN
20   VALASPLYSGLUGLUGLUALAVALTHRILE
21   GLUMETASNGLUPROVALGLNLEUTHRPHE
22   ALALEUARGTYRLEUASNPHEPHETHRLYS
23   ALATHRPROLEUSERSERTHRVALTHRLEU
24   SERMETSERALAASPVALPROLEUVALVAL
25   GLUTYRLYSILEALAASPMETGLYHISLEU
26   LYSTYRTYRLEUALAPROLYSILEGLUASP
27   GLUGLUGLYSER

Samples:

sample_1: PCNA 0.1 mM; P15(50-77) 0.64 mM; DNA duplex 0.1 mM; sodium phosphate 20 ± 0.1 mM; sodium chloride 50 ± 0.1 mM

sample_conditions_1: ionic strength: 0.06 M; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D-1H-15N-TROSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks