BMRB Entry 27557

Name: NMR Assignment of Backbone (1H, 15N, and 13C) resonances of Histone Like DNA binding protein of Helicobacter pylori (Hup) at low pH
Published: 2018-12-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27557

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR Assignment of Backbone (1H, 15N, and 13C) resonances of Histone Like DNA binding protein of Helicobacter pylori (Hup) at low pH

Deposition date:

2018-07-25

Original release date:

2018-12-12

Authors:

Agarwal, Nipanshu; Jaiswal, Nancy; Kumar, Dinesh; Poluri, Krishna Mohan

Citation:

Citation: Jaiswal, Nancy; Agarwal, Nipanshu; Kaur, Anupreet; Tripathi, Sarita; Gahlay, Gagandeep Kaur; Arora, Ashish; Mitthu, Venus Singh; Poluri, Krishna Mohan; Kumar, Dinesh. "Molecular interaction between human SUMO-I and histone like DNA binding protein of Helicobacter pylori (Hup) investigated by NMR and other biophysical tools" Int. J. Biol. Macromol. 123, 446-456 (2018).
PubMed: 30439429

Assembly members:

Assembly members:
Histone_like_DNA_Binding_Protein_of_Helicobacter_pylori, polymer, 98 residues, 10772 Da.

Natural source:

Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE80L

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Histone_like_DNA_Binding_Protein_of_Helicobacter_pylori: GAMEMNKAEFIDLVKEAGKY NSKREAEEAISAFTLAVETA LSKGESVELIGFGKFETAEQ KGKEGKVPGSDKTYKTEDKR VPKFKPGKTLKQKVEEGK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	483
15N chemical shifts	168
1H chemical shifts	168

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hup A	1
2	Hup B	1

Entities:

Entity 1, Hup A 98 residues - 10772 Da.

Residue -4 to -1 represent cloning artifacts

1

GLY

ALA

MET

GLU

MET

ASN

LYS

ALA

GLU

PHE

2

ILE

ASP

LEU

VAL

LYS

GLU

ALA

GLY

LYS

TYR

3

ASN

SER

LYS

ARG

GLU

ALA

GLU

ALA

ILE

4

SER

ALA

PHE

THR

LEU

ALA

VAL

GLU

THR

ALA

5

LEU

SER

LYS

GLY

GLU

SER

VAL

GLU

LEU

ILE

6

GLY

PHE

GLY

LYS

PHE

GLU

THR

ALA

GLU

GLN

7

LYS

GLY

LYS

GLU

GLY

LYS

VAL

PRO

GLY

SER

8

ASP

LYS

THR

TYR

LYS

THR

GLU

ASP

LYS

ARG

9

VAL

PRO

LYS

PHE

LYS

PRO

GLY

LYS

THR

LEU

10

LYS

GLN

LYS

VAL

GLU

GLY

LYS

Samples:

sample_1: Histone like DNA Binding Protein of Helicobacter pylori (Hup), [U-99% 13C; U-99% 15N], 0.8 mM; D2O, [U-100% 2H], 10%; H2O 90%; sodium azide 0.1%; sodium chloride 300 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.079 M; pH: 1.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.079 M; pH: 1.5; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1, Freely Available - chemical shift assignment, data analysis, structure solution

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks