BMRB Entry 27546

Title:
Aotus trivirgatus Eosinophil-Derived Neurotoxin backbone assignment
Deposition date:
2018-07-13
Original release date:
2018-07-19
Authors:
Bernard, David; Doucet, Nicolas
Citation:

Citation: Bernard, David; Narayanan, Chitra; Bafna, Khushboo; Bhojane, Purva; Letourneau, Myriam; Howell, Elizabeth; Agarwal, Pratul; Doucet, Nicolas. "Aotus trivirgatus Eosinophil-Derived Neurotoxin backbone assignment"  .

Assembly members:

Assembly members:
AtEDN, polymer, 131 residues, 14857 Da.

Natural source:

Natural source:   Common Name: douroucouli   Taxonomy ID: 9505   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aotus trivirgatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJexpress 414

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts119
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Aotus trivirgatus EDN1

Entities:

Entity 1, Aotus trivirgatus EDN 131 residues - 14857 Da.

1   ALAPROGLNLYSPHETHRARGALAGLNTRP
2   PHESERILEGLNHISILEGLNTHRTHRPRO
3   LEUARGCYSTHRASNALAMETARGALAILE
4   ASNLYSTYRGLNHISARGCYSLYSASNGLN
5   ASNTHRPHELEUHISTHRTHRPHEALAALA
6   VALVALASNVALCYSGLYASNTHRASNILE
7   THRCYSPROARGASNALASERLEUASNASN
8   CYSHISHISSERARGVALGLNVALPROLEU
9   THRTYRCYSASNLEUTHRGLYPROPROTHR
10   ILETHRASNCYSVALTYRSERSERTHRGLN
11   ALAASNMETPHETYRVALVALALACYSASP
12   ASNARGASPGLNARGASPPROPROGLNTYR
13   PROVALVALPROVALHISLEUASPTHRTHR
14   ILE

Samples:

sample_1: AtEDN, [U-99% 13C; U-99% 15N], 300 uM; sodium acetate 15 mM

sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CcpNmr v2.4, CCPN - peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP O18937
EMBL AAB71338.1
AlphaFold O18937

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks