BMRB Entry 27536

Name: 1H, 13C and 15N chemical shift assignments for carbohydrate binding module x (CBMx)
Published: 2018-10-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27536

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignments for carbohydrate binding module x (CBMx)

Deposition date:

2018-07-06

Original release date:

2018-10-02

Authors:

Madland, Eva; Aachmann, Finn

Citation:

Citation: Madland, Eva; Kitaoku, Yoshihito; Saetrom, Gerd Inger; Leth, Maria Louise; Ejby, Morten; Hachem, Maher Abou; Aachmann, Finn Lillelund. "1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis" Biomol. NMR Assignments 13, 55-58 (2018).
PubMed: 30244308

Assembly members:

Assembly members:
CBMx, polymer, 141 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBMx: GAMGVKKVFTADQLKVAWGD ADYELADGQWKLSFAKQYNQ VKWTLPESIEMSQVNAVTFQ VADQKVPISLKVYNGGDDAT AANTQYGLSGQTEYTINPSG DGAIDAVGIMITEDKPENAT VSLVSVTFELKAGAGDAKLG D

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	543
15N chemical shifts	135
1H chemical shifts	867

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CBMx	1

Entities:

Entity 1, CBMx 141 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

VAL

LYS

VAL

PHE

THR

2

ALA

ASP

GLN

LEU

LYS

VAL

ALA

TRP

GLY

ASP

3

ALA

ASP

TYR

GLU

LEU

ALA

ASP

GLY

GLN

TRP

4

LYS

LEU

SER

PHE

ALA

LYS

GLN

TYR

ASN

GLN

5

VAL

LYS

TRP

THR

LEU

PRO

GLU

SER

ILE

GLU

6

MET

SER

GLN

VAL

ASN

ALA

VAL

THR

PHE

GLN

7

VAL

ALA

ASP

GLN

LYS

VAL

PRO

ILE

SER

LEU

8

LYS

VAL

TYR

ASN

GLY

ASP

ALA

THR

9

ALA

ASN

THR

GLN

TYR

GLY

LEU

SER

GLY

10

GLN

THR

GLU

TYR

THR

ILE

ASN

PRO

SER

GLY

11

ASP

GLY

ALA

ILE

ASP

ALA

VAL

GLY

ILE

MET

12

ILE

THR

GLU

ASP

LYS

PRO

GLU

ASN

ALA

THR

13

VAL

SER

LEU

VAL

SER

VAL

THR

PHE

GLU

LEU

14

LYS

ALA

GLY

ALA

GLY

ASP

ALA

LYS

LEU

GLY

15

ASP

Samples:

15N_sample: CBMx, [U-98% 15N], 1 mM; sodium phosphate 50 mM

13C_15N_sample: CBMx, [U-98% 13C; U-98% 15N], 1.7 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.11 M; pH: 6.5; pressure: 1 atm; temperature: 298.1 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	13C_15N_sample	isotropic	sample_conditions_1
3D HNCA	13C_15N_sample	isotropic	sample_conditions_1
3D HNCO	13C_15N_sample	isotropic	sample_conditions_1
3D HN(CO)CA	13C_15N_sample	isotropic	sample_conditions_1
3D HNCACB	13C_15N_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	13C_15N_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	13C_15N_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	13C_15N_sample	isotropic	sample_conditions_1
3D HN(CA)CO	13C_15N_sample	isotropic	sample_conditions_1
3D HBHANH	13C_15N_sample	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection

CARA v1.5, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks