BMRB Entry 27518

Title:
chemical shifts assignments of Nb26 against aflatoxin B1
Deposition date:
2018-06-12
Original release date:
2018-07-19
Authors:
Nie, Yao; He, Ting; Zhu, Jiang; Yang, Yunhuang
Citation:

Citation: Nie, Yao; Li, Shuangli; Zhu, Jiang; Hu, Rui; Liu, Maili; He, Ting; Yang, Yunhuang. "Chemical shift assignments of a camelid nanobody against aflatoxin B"  Biomol NMR Assign 13, 75-78 (2019).
PubMed: 30328057

Assembly members:

Assembly members:
Nb26, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Alpaca   Taxonomy ID: 30538   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Vicugna pacos

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32m

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts127
1H chemical shifts729

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nb261

Entities:

Entity 1, Nb26 131 residues - Formula weight is not available

1   METGLNLEUGLNLEUVALGLUSERGLYGLY
2   GLYLEUVALGLNALAGLYGLYSERLEUARG
3   LEUSERCYSALAALASERGLYARGTHRPHE
4   SERSERTYRALAMETGLYTRPPHEARGGLN
5   ALAPROGLYLYSGLUARGGLUPHEVALALA
6   VALVALASNTRPSERGLYARGARGTHRTYR
7   TYRALAASPSERVALLYSGLYARGPHETHR
8   ILESERARGASPASNALALYSASNTHRVAL
9   TYRLEUGLNMETASNSERLEULYSPROGLU
10   ASPTHRALAVALTYRASNCYSALAALAGLY
11   LYSTRPASPGLYSERTYRTYRGLYALAPRO
12   ASPTYRTRPGLYGLNGLYTHRGLNVALTHR
13   VALSERSERLEUGLUHISHISHISHISHIS
14   HIS

Samples:

sample_1: Nb26, [U-99% 13C; U-99% 15N], 0.75 mM

sample_2: Nb26, [U-99% 13C; U-99% 15N], 0.45 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks