BMRB Entry 27512

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27512

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Title:
Backbone resonance ssignment of the human ubiquitin G76C mutant
Deposition date:
2018-06-06
Original release date:
2018-07-12
Authors:
Wiesner, Silke
Citation:

Citation: Jaeckl, Magnus; Stollmaier, Carsten; Strohaeker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona; Wiesner, Silke. "Beta-Sheet Augmentation Is a Conserved Mechanism of Priming HECT E3 Ligases for Ubiquitin Ligation"  J. Mol. Biol. 430, 3218-3233 (2018).
PubMed: 29964046

Assembly members:

Assembly members:
ub, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-M11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ub: GAMGMQIFVKTLTGKTITLE VEPSDTIENVKAKIQDKEGI PPDQQRLIFAGKQLEDGRTL SDYNIQKESTLHLVLRLRGC

Data sets:
Data typeCount
13C chemical shifts142
15N chemical shifts70
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ub1

Entities:

Entity 1, ub 80 residues - Formula weight is not available

1   GLYALAMETGLYMETGLNILEPHEVALLYS
2   THRLEUTHRGLYLYSTHRILETHRLEUGLU
3   VALGLUPROSERASPTHRILEGLUASNVAL
4   LYSALALYSILEGLNASPLYSGLUGLYILE
5   PROPROASPGLNGLNARGLEUILEPHEALA
6   GLYLYSGLNLEUGLUASPGLYARGTHRLEU
7   SERASPTYRASNILEGLNLYSGLUSERTHR
8   LEUHISLEUVALLEUARGLEUARGGLYCYS

Samples:

sample_1: ub, [U-100% 13C; U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; D2O, [U-99% 2H], 10 % w/v

sample_conditions_1: ionic strength: 0.2 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks