BMRB Entry 27494

Title:
Backbone 1H, 13C, 15N chemical shifts of deubiquitinase A
Deposition date:
2018-05-29
Original release date:
2020-02-28
Authors:
Kabra, Ashish; Benson, Catherine; Li, Ying
Citation:

Citation: Kabra, Ashish; Benson, Catherine; Li, Ying. "Backbone 1 H, 13 C, and 15 N resonance assignments of deubiquitinase A in non-phosphorylated and phosphorylated forms"  Biomol. NMR Assign. 13, 37-42 (2019).
PubMed: 30232733

Assembly members:

Assembly members:
Deubiquitinase_A, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28-MHL

Data sets:
Data typeCount
13C chemical shifts359
15N chemical shifts114
1H chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Deubiquitinase A1

Entities:

Entity 1, Deubiquitinase A 168 residues - Formula weight is not available

1   GLYALAGLYTYRASNSERGLUASPGLUTYR
2   GLUALAALAALAALAARGILEGLUALAMET
3   ASPPROALATHRVALGLUGLNGLNGLUHIS
4   TRPPHEGLULYSALALEUARGASPLYSLYS
5   GLYPHEILEILELYSGLNMETLYSGLUASP
6   GLYALACYSLEUPHEARGALAVALALAASP
7   GLNVALTYRGLYASPGLNASPMETHISGLU
8   VALVALARGLYSHISCYSMETASPTYRLEU
9   METLYSASNALAASPTYRPHESERASNTYR
10   VALTHRGLUASPPHETHRTHRTYRILEASN
11   ARGLYSARGLYSASNASNCYSHISGLYASN
12   HISILEGLUMETGLNALAMETALAGLUMET
13   TYRASNARGPROVALGLUVALTYRGLNTYR
14   SERTHRGLUPROILEASNTHRPHEHISGLY
15   ILEHISGLNASNGLUASPGLUPROILEARG
16   VALSERTYRHISARGASNILEHISTYRASN
17   SERVALVALASNPROASNLYSALA

Samples:

sample_1: Deubiquitinase A, [U-13C; U-15N; U-2H], 0.5 mM; sodium phosphate 50 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian VNMRS 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks