BMRB Entry 27491
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27491
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Citation: Boros, Eszter; Sebak, Fanni; Heja, David; Szakacs, David; Zboray, Katalin; Schlosser, Gitta; Micsonai, Andras; Kardos, Jozsef; Bodor, Andrea; Pal, Gabor. "Directed Evolution of Canonical Loops and Their Swapping between Unrelated Serine Proteinase Inhibitors Disprove the Interscaffolding Additivity Model" J. Mol. Biol. 431, 557-575 (2019).
PubMed: 30543823
Assembly members:
SGPI-2_variant, polymer, 38 residues, 4008 Da.
Natural source: Common Name: desert locust Taxonomy ID: 7010 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Schistocerca gregaria
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: M13KO7
Entity Sequences (FASTA):
SGPI-2_variant: GSGEVTCEPGTTFKDKCNTC
RCGSDGKSADCTMKMCKQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 170 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SGPI-2 variant | 1 |
Entities:
Entity 1, SGPI-2 variant 38 residues - 4008 Da.
Residues -2-0 represent a cloning artifact
| 1 | GLY | SER | GLY | GLU | VAL | THR | CYS | GLU | PRO | GLY | ||||
| 2 | THR | THR | PHE | LYS | ASP | LYS | CYS | ASN | THR | CYS | ||||
| 3 | ARG | CYS | GLY | SER | ASP | GLY | LYS | SER | ALA | ASP | ||||
| 4 | CYS | THR | MET | LYS | MET | CYS | LYS | GLN |
Samples:
sample_1: SGPI-2 variant 1 mM; MES 10 mM; sodium chloride 50 mM; DSS 5 uL
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz