BMRB Entry 27477

Title:
The complete 1H, 13C, 15N chemical shift assignment of the Itch HECT C-lobe
Deposition date:
2018-05-10
Original release date:
2018-10-03
Authors:
Steven, Beasley; Roela, Bardhi; Donald, Spratt
Citation:

Citation: Beasley, Steven; Bardhi, Roela; Spratt, Donald. "1H, 13C, and 15N resonance assignments of the C-terminal lobe of the human HECT E3 ubiquitin ligase ITCH"  Biomol. NMR Assignments 13, 15-20 (2019).
PubMed: 30229450

Assembly members:

Assembly members:
ITCH_C-lobe, polymer, 122 residues, 14402.39 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUC derivative

Data typeCount
13C chemical shifts571
15N chemical shifts130
1H chemical shifts869

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ITCH C-lobe1

Entities:

Entity 1, ITCH C-lobe 122 residues - 14402.39 Da.

The first 2 residues are a cloning artifact after affinity tag cleavage; G784-E903 is the remainder based on Uniprot Q96J02.

1   GLYSERGLYMETGLNGLUILEASPLEUASN
2   ASPTRPGLNARGHISALAILETYRARGHIS
3   TYRALAARGTHRSERLYSGLNILEMETTRP
4   PHETRPGLNPHEVALLYSGLUILEASPASN
5   GLULYSARGMETARGLEULEUGLNPHEVAL
6   THRGLYTHRSERARGLEUPROVALGLYGLY
7   PHEALAASPLEUMETGLYSERASNGLYPRO
8   GLNLYSPHESERILEGLULYSVALGLYLYS
9   GLUASNTRPLEUPROARGSERHISTHRCYS
10   PHEASNARGLEUASPLEUPROPROTYRLYS
11   SERTYRGLUGLNLEULYSGLULYSLEULEU
12   PHEALAILEGLUGLUTHRGLUGLYPHEGLY
13   GLNGLU

Samples:

sample_1: ITCH C-lobe, [U-100% 13C; U-100% 15N], 1.75 mM; MES 20 mM; sodium chloride 120 mM; EDTA 1 mM; TCEP 2 mM

sample_2: ITCH C-lobe, [U-100% 13C; U-100% 15N], 1.75 mM; MES 20 mM; sodium chloride 120 mM; EDTA 1 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 143 mM; pH: 6.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRView v9.1.0-b63, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking, processing, refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

UNP Q96J02
AlphaFold Q9H4U5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks