BMRB Entry 27438

Name: Competitive Regulation of the Cold Sensing Ion Channel TRPM8 by PIP2 and PIRT
Published: 2019-04-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27438

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Competitive Regulation of the Cold Sensing Ion Channel TRPM8 by PIP2 and PIRT

Deposition date:

2018-03-29

Original release date:

2019-04-12

Authors:

Sisco, Nicholas; Helsel, Cole; Rath, Parthasarathi; Machorro, Dorothy; Van Horn, Wade

Citation:

Citation: Hilton, Jacob; Salehpour, Taraneh; Sisco, Nicholas; Rath, Parthasarathi; Van Horn, Wade. "Phosphoinositide-interacting regulator of TRP (PIRT) has opposing effects on human and mouse TRPM8 ion channels" J. Biol. Chem. 293, 9423-9434 (2018).
PubMed: 29724821

Assembly members:

Assembly members:
Human_PIRT, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human_PIRT: MGHHHHHHGMTMETLPKVLE VDEKSPEAKDLLPSQTASSL CISSRSESVWTTTPRSNWEI YRKPIVIMSVGGAILLFGVV ITCLAYTLKLSDKSLSILKM VGPGFLSLGLMMLVCGLVWV PIIKKKQKHRQKSNFLRSLK SFFLTR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	141
15N chemical shifts	91
1H chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human PIRT	1

Entities:

Entity 1, Human PIRT 146 residues - Formula weight is not available

1

MET

GLY

HIS

GLY

MET

2

THR

MET

GLU

THR

LEU

PRO

LYS

VAL

LEU

GLU

3

VAL

ASP

GLU

LYS

SER

PRO

GLU

ALA

LYS

ASP

4

LEU

PRO

SER

GLN

THR

ALA

SER

LEU

5

CYS

ILE

SER

ARG

SER

GLU

SER

VAL

TRP

6

THR

PRO

ARG

SER

ASN

TRP

GLU

ILE

7

TYR

ARG

LYS

PRO

ILE

VAL

ILE

MET

SER

VAL

8

GLY

ALA

ILE

LEU

PHE

GLY

VAL

9

ILE

THR

CYS

LEU

ALA

TYR

THR

LEU

LYS

LEU

10

SER

ASP

LYS

SER

LEU

SER

ILE

LEU

LYS

MET

11

VAL

GLY

PRO

GLY

PHE

LEU

SER

LEU

GLY

LEU

12

MET

LEU

VAL

CYS

GLY

LEU

VAL

TRP

VAL

13

PRO

ILE

LYS

GLN

LYS

HIS

ARG

14

GLN

LYS

SER

ASN

PHE

LEU

ARG

SER

LEU

LYS

15

SER

PHE

LEU

THR

ARG

Samples:

sample_1: Human PIRT, [U-100% 13C; U-100% 15N], 500-750 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks