BMRB Entry 27411

Title:
1H, 13C and 15N chemical shift assignments for a family AA9 LPMO from Thermoascus aurantiacus (TaLPMO9A)
Deposition date:
2018-02-27
Original release date:
2018-09-19
Authors:
Kitaoku, Yoshihito; Courtade, Gaston; Petrovic, Dejan; Eijsink, Vincent; Aachmann, Finn
Citation:

Citation: Kitaoku, Yoshihito; Courtade, Gaston; Petrovic, Dejan; Fukamizo, Tamo; Eijsink, Vincent; Aachmann, Finn. "Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase TaLPMO9A"  Biomol. NMR Assignments 12, 357-361 (2018).
PubMed: 30117034

Assembly members:

Assembly members:
TaLPMO9A, polymer, 228 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermoascus aurantiacus   Taxonomy ID: 5087   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Thermoascus aurantiacus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPink-GAP

Data sets:
Data typeCount
13C chemical shifts725
15N chemical shifts196
1H chemical shifts1065

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TaLPMO9A1

Entities:

Entity 1, TaLPMO9A 228 residues - Formula weight is not available

1   HISGLYPHEVALGLNASNILEVALILEASP
2   GLYLYSASNTYRGLYGLYTYRLEUVALASN
3   GLNTYRPROTYRMETSERASNPROPROGLU
4   VALILEALATRPSERTHRTHRALATHRASP
5   LEUGLYPHEVALASPGLYTHRGLYTYRGLN
6   THRPROASPILEILECYSHISARGGLYALA
7   LYSPROGLYALALEUTHRALAPROVALSER
8   PROGLYGLYTHRVALGLULEUGLNTRPTHR
9   PROTRPPROASPSERHISHISGLYPROVAL
10   ILEASNTYRLEUALAPROCYSASNGLYASP
11   CYSSERTHRVALASPLYSTHRGLNLEUGLU
12   PHEPHELYSILEALAGLUSERGLYLEUILE
13   ASNASPASPASNPROPROGLYILETRPALA
14   SERASPASNLEUILEALAALAASNASNSER
15   TRPTHRVALTHRILEPROTHRTHRILEALA
16   PROGLYASNTYRVALLEUARGHISGLUILE
17   ILEALALEUHISSERALAGLNASNGLNASP
18   GLYALAGLNASNTYRPROGLNCYSILEASN
19   LEUGLNVALTHRGLYGLYGLYSERASPASN
20   PROALAGLYTHRLEUGLYTHRALALEUTYR
21   HISASPTHRASPPROGLYILELEUILEASN
22   ILETYRGLNLYSLEUSERSERTYRILEILE
23   PROGLYPROPROLEUTYRTHRGLY

Samples:

sample_H2O: TaLPMO9A, [U-98% 13C; U-98% 15N], 50 uM; ammonium acetate 25 mM

sample_D2O: TaLPMO9A, [U-98% 13C; U-98% 15N], 50 uM; sodium phosphate 50 mM; sodium chloride 25 mM

sample_conditions_H2O: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_D2O: ionic strength: 75 mM; pH*: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_H2Oisotropicsample_conditions_H2O
3D 1H-15N NOESYsample_H2Oisotropicsample_conditions_H2O
3D CBCA(CO)NHsample_H2Oisotropicsample_conditions_H2O
3D HBHA(CO)NHsample_H2Oisotropicsample_conditions_H2O
3D HCCH-TOCSYsample_D2Oisotropicsample_conditions_D2O
3D HNCACBsample_H2Oisotropicsample_conditions_H2O
3D HN(CA)COsample_H2Oisotropicsample_conditions_H2O
3D HNCAsample_H2Oisotropicsample_conditions_H2O
3D HNCOsample_H2Oisotropicsample_conditions_H2O
2D 1H-13C HSQC aliphaticsample_D2Oisotropicsample_conditions_D2O
2D 1H-13C HSQC aromaticsample_D2Oisotropicsample_conditions_D2O
3D 1H-13C NOESY aromaticsample_D2Oisotropicsample_conditions_D2O

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

CARA v1.5/1.8, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Ascend 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks