BMRB Entry 27382

Title:
mu-PIIIA-4
Deposition date:
2018-01-23
Original release date:
2018-02-23
Authors:
Heimer, Pascal; Tietze, Daniel; Tietze, Alesia; Ohlenschlaeger, Oliver; Imhof, Diana
Citation:

Citation: Heimer, Pascal; Tietze, Alesia; Baeuml, Charlotte; Resemann, Anja; Mayer, Franz; Suckau, Detlev; Ohlenschlaeger, Oliver; Tietze, Daniel; Imhof, Diana. "Conformational mu-Conotoxin PIIIA Isomers Revisited: Impact of Cysteine Pairing on Disulfide-Bond Assignment and Structure Elucidation"  Anal. Chem. 90, 3321-3327 (2018).
PubMed: 29397705

Assembly members:

Assembly members:
conotoxin_muPIIIA-4, polymer, 23 residues, 2603.1 Da.

Natural source:

Natural source:   Common Name: Conus purpurascens   Taxonomy ID: 41690   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus purpurascens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
conotoxin_muPIIIA-4: XRLCCGFXKSCRSRQCKXHR CCX

Data sets:
Data typeCount
1H chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1conotoxin_muPIIIA-41

Entities:

Entity 1, conotoxin_muPIIIA-4 23 residues - 2603.1 Da.

Residue 1: Pyrrolidone carboxylic acid Residues 8, 18: 4-hydroxyproline Residue 22: cysteine amide

1   PCAARGLEUCYSCYSGLYPHEHYPLYSSER
2   CYSARGSERARGGLNCYSLYSHYPHISARG
3   CYSCYSNH2

Samples:

sample_1: conotoxin_muPIIIA-4 0.5 ± 0.2 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker - collection

CCPNMR v2.4, CCPN - chemical shift assignment

Yasara, Yasara Biosciences GmbH - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz