BMRB Entry 27375

Title:
1H, 13C and 15N backbone resonance assignment of progerin C-terminal region without Cysteines
Deposition date:
2018-01-22
Original release date:
2018-01-26
Authors:
Celli, Florian; Petitalot, Ambre; Samson, Camille; Theillet, Francois-Xavier; Zinn-Justin, Sophie
Citation:

Citation: Celli, Florian; Petitalot, Ambre; Samson, Camille; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region specific to prelamin A"  Biomol. NMR Assignments 12, 225-229 (2018).
PubMed: 29582385

Assembly members:

Assembly members:
progCterNoCys, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
progCterNoCys: GSHASSSGDPAEYNLRSRTV LAGTAGQPADKASASGSGAQ SPQNASIM

Data sets:
Data typeCount
13C chemical shifts127
15N chemical shifts44
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Progerin C-terminal region1

Entities:

Entity 1, Progerin C-terminal region 48 residues - Formula weight is not available

1   GLYSERHISALASERSERSERGLYASPPRO
2   ALAGLUTYRASNLEUARGSERARGTHRVAL
3   LEUALAGLYTHRALAGLYGLNPROALAASP
4   LYSALASERALASERGLYSERGLYALAGLN
5   SERPROGLNASNALASERILEMET

Samples:

sample_1: progCterNoCys, [U-100% 13C; U-100% 15N], 600 uM; Phosphate Buffer, Natural Abundance, 20 mM; NaCl, Natural Abundance, 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)NNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks