BMRB Entry 27365

Title:
CSP1_DPC
Deposition date:
2018-01-18
Original release date:
2019-02-08
Authors:
Yang, Yifang; Cornilescu, Gabriel; Tal-Gan, Yftah
Citation:

Citation: Yang, Yifang; Cornilescu, Gabriel; Tal-Gan, Yftah. "Structural Characterization of Competence-Stimulating Peptide Analogues Reveals Key Features for ComD1 and ComD2 Receptor Binding in Streptococcus pneumoniae"  Biochemistry 57, 5359-5369 (2018).
PubMed: 30125091

Assembly members:

Assembly members:
CSP1, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptococcus pneumoniae   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: streptococcus pneumoniae

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CSP1: EMRLSKFFRDFILQRKK

Data sets:
Data typeCount
13C chemical shifts32
15N chemical shifts18
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CSP1, 11
2CSP1, 21

Entities:

Entity 1, CSP1, 1 17 residues - Formula weight is not available

1   GLUMETARGLEUSERLYSPHEPHEARGASP
2   PHEILELEUGLNARGLYSLYS

Samples:

sample_1: DPC, [U-2H], 250 mM; CSP1 1.9 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

X-PLOR_NIH vv. 2.42, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks