BMRB Entry 27326

Title:
Yes1 SH2 domain in complex with Cdc37-derived phosphopeptide
Deposition date:
2017-12-03
Original release date:
2018-01-16
Authors:
Bachman, Ashleigh; Keramisanou, Dimitra; Kumar M. V., Vasantha; Gelis, Ioannis
Citation:

Citation: Bachman, Ashleigh; Keramisanou, Dimitra; Xu, Wanping; Beebe, Kristin; Moses, Michael; Vasantha Kumar, M; Gray, Geoffrey; Noor, Radwan Ebna; van der Vaart, Arjan; Neckers, Len; Gelis, Ioannis. "Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation"  Nat. Commun. 9, 265-265 (2018).
PubMed: 29343704

Assembly members:

Assembly members:
Yes1-SH2, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6P1

Data sets:
Data typeCount
13C chemical shifts204
15N chemical shifts107
1H chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH2 domain1

Entities:

Entity 1, SH2 domain 111 residues - Formula weight is not available

1   SERILEGLNALAGLUGLUTRPTYRPHEGLY
2   LYSMETGLYARGLYSASPALAGLUARGLEU
3   LEULEUASNPROGLYASNGLNARGGLYILE
4   PHELEUVALARGGLUSERGLUTHRTHRLYS
5   GLYALATYRSERLEUSERILEARGASPTRP
6   ASPGLUILEARGGLYASPASNVALLYSHIS
7   TYRLYSILEARGLYSLEUASPASNGLYGLY
8   TYRTYRILETHRTHRARGALAGLNPHEASP
9   THRLEUGLNLYSLEUVALLYSHISTYRTHR
10   GLUHISALAASPGLYLEUCYSHISLYSLEU
11   THRTHRVALCYSPROTHRVALLYSPROGLN
12   GLY

Samples:

sample_1: Yes1-SH2, [U-100% 13C; U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Agilent direct drive 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks