BMRB Entry 27240

Title:
NMR backbone and side chain assignments of the Kringle domain of ROR1
Deposition date:
2017-08-30
Original release date:
2017-10-31
Authors:
Ma, Xiaofang; Hu, Kaifeng
Citation:

Citation: Ma, Xiaofang; Zhang, Yingying; Liu, Bin; Yang, Jiahui; Hu, Kaifeng. "Backbone and side-chain chemical shift assignments of the kringle domain of human receptor tyrosine kinase-like orphan receptor 1 (ROR1)"  Biomol. NMR Assign. 12, 145-148 (2018).
PubMed: 29313214

Assembly members:

Assembly members:
Kringle_domain_of_ROR1, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts331
15N chemical shifts84
1H chemical shifts512

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ROR1 Kringle monomer1

Entities:

Entity 1, ROR1 Kringle monomer 85 residues - Formula weight is not available

1   GLYSERLYSCYSTYRASNSERTHRGLYVAL
2   ASPTYRARGGLYTHRVALSERVALTHRLYS
3   SERGLYARGGLNCYSGLNPROTRPASNSER
4   GLNTYRPROHISTHRHISTHRPHETHRALA
5   LEUARGPHEPROGLULEUASNGLYGLYHIS
6   SERTYRCYSARGASNPROGLYASNGLNLYS
7   GLUALAPROTRPCYSPHETHRLEUASPGLU
8   ASNPHELYSSERASPLEUCYSASPILEPRO
9   ALACYSASPSERLYS

Samples:

sample_1: Kringle domain of ROR1, [U-99% 13C; U-99% 15N], 0.8 mM

sample_conditions_1: ionic strength: 150 mM; pH: 8.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-13C CT-HSQC aliphaticsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSY aromaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks