BMRB Entry 27231

Title:
Backbone assignments of the full length Dengue Virus NS4A protein in micelles
Deposition date:
2017-08-23
Original release date:
2017-11-03
Authors:
Li, Yan; Lee, Michelle Yueqi; Kang, CongBao
Citation:

Citation: Li, Yan; Lee, Michelle Yueqi; Loh, Ying Ru; Kang, CongBao. "Secondary structure and membrane topology of dengue virus NS4A protein in micelles"  Biochim. Biophys. Acta S0005-2736, 30334-30336 (2017).
PubMed: 29055659

Assembly members:

Assembly members:
full_length_Dengue_NS4A, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Dengue Virus   Taxonomy ID: 12637   Superkingdom: Viruses   Kingdom: not available   Genus/species: Dengue Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts117
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1full length Dengue NS4A1

Entities:

Entity 1, full length Dengue NS4A 136 residues - Formula weight is not available

1   METSERILETHRLEUASPILELEUTHRGLU
2   ILEALASERLEUPROTHRTYRLEUSERSER
3   ARGALALYSLEUALALEUASPASNILEVAL
4   METLEUHISTHRTHRGLUARGGLYGLYARG
5   ALATYRGLNHISALALEUASNGLULEUPRO
6   GLUSERLEUGLUTHRLEUMETLEUVALALA
7   LEULEUGLYALAMETTHRALAGLYILEPHE
8   LEUPHEPHEMETGLNGLYLYSGLYILEGLY
9   LYSLEUSERMETGLYLEUILETHRILEALA
10   VALALASERGLYLEULEUTRPVALALAGLU
11   ILEGLNPROGLNTRPILEALAALASERILE
12   ILELEUGLUPHEPHELEUMETVALLEULEU
13   ILEPROGLUPROGLULYSGLNARGLEUGLU
14   HISHISHISHISHISHIS

Samples:

sample_1: full_length_Dengue_NS4A, [U-13C; U-15N; U-2H], 0.5 mM; sodium phosphate 20 mM; DPC 200 mM; DTT 1 mM; sodium chloride 70 mM

sample_conditions_1: ionic strength: 90 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Keller and Wuthrich - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks