BMRB Entry 27214

Title:
RSK1 phosphorylated C-terminal peptide (696-735)
Deposition date:
2017-08-10
Original release date:
2017-10-31
Authors:
Gogl, Gergo; Nyitray, Laszlo
Citation:

Citation: Gogl, Gergo; Biri-Kovacs, Beata; Poti, Adam Levente; Vadaszi, Henrietta; Szeder, Balint; Bodor, Andrea; Schlosser, Gitta; Acs, Andras; Turiak, Lilla; Buday, Laszlo; Alexa, Anita; Nyitray, Laszlo; Remenyi, Attila. "Dynamic control of RSK complexes by phosphoswitch-based regulation"  FEBS J. 85, 46-71 (2017).
PubMed: 29083550

Assembly members:

Assembly members:
RSK1_peptide, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: petara

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RSK1_peptide: GSGAMAATYSALNSSKPTPQ LKPIESSILAQRRVRKLPXT TL

Data sets:
Data typeCount
15N chemical shifts35
1H chemical shifts35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RSK1 peptide1

Entities:

Entity 1, RSK1 peptide 42 residues - Formula weight is not available

1   GLYSERGLYALAMETALAALATHRTYRSER
2   ALALEUASNSERSERLYSPROTHRPROGLN
3   LEULYSPROILEGLUSERSERILELEUALA
4   GLNARGARGVALARGLYSLEUPROSEPTHR
5   THRLEU

Samples:

sample_1: RSK1 peptide, [U-99% 13C; U-99% 15N], 0.7 mM; sodium chloride 20 mM; HEPES 20 mM; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.75; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

Sparky, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks