BMRB Entry 27211

Title:
Solid-state NMR assignment of P. horikoshii TET2
Deposition date:
2017-08-09
Original release date:
2017-10-31
Authors:
Sivertsen, Astrid; Schanda, Paul
Citation:

Citation: Gauto, Diego; Estrozi, Leandro; Schwieters, Charles; Effantin, Gregory; Macek, Pavel; Sounier, Remy; Sivertsen, Astrid; Schmidt, Elena; Kerfah, Rime; Mas, Guillaume; Colletier, Jacques-Philippe; Guntert, Peter; Favier, Adrien; Schoehn, Guy; Schanda, Paul; Boisbouvier, Jerome. "Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex"  Nat. Commun. 10, 2697-2697 (2019).
PubMed: 31217444

Assembly members:

Assembly members:
TET, polymer, 353 residues, 39015.9784 Da.

Natural source:

Natural source:   Common Name: Pyrococcus horikoshii   Taxonomy ID: 53953   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Pyrococcus horikoshii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Data sets:
Data typeCount
13C chemical shifts1080
15N chemical shifts289
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TET1

Entities:

Entity 1, TET 353 residues - 39015.9784 Da.

1   METGLUVALARGASNMETVALASPTYRGLU
2   LEULEULYSLYSVALVALGLUALAPROGLY
3   VALSERGLYTYRGLUPHELEUGLYILEARG
4   ASPVALVALILEGLUGLUILELYSASPTYR
5   VALASPGLUVALLYSVALASPLYSLEUGLY
6   ASNVALILEALAHISLYSLYSGLYGLUGLY
7   PROLYSVALMETILEALAALAHISMETASP
8   GLNILEGLYLEUMETVALTHRHISILEGLU
9   LYSASNGLYPHELEUARGVALALAPROILE
10   GLYGLYVALASPPROLYSTHRLEUILEALA
11   GLNARGPHELYSVALTRPILEASPLYSGLY
12   LYSPHEILETYRGLYVALGLYALASERVAL
13   PROPROHISILEGLNLYSPROGLUASPARG
14   LYSLYSALAPROASPTRPASPGLNILEPHE
15   ILEASPILEGLYALAGLUSERLYSGLUGLU
16   ALAGLUASPMETGLYVALLYSILEGLYTHR
17   VALILETHRTRPASPGLYARGLEUGLUARG
18   LEUGLYLYSHISARGPHEVALSERILEALA
19   PHEASPASPARGILEALAVALTYRTHRILE
20   LEUGLUVALALALYSGLNLEULYSASPALA
21   LYSALAASPVALTYRPHEVALALATHRVAL
22   GLNGLUGLUVALGLYLEUARGGLYALAARG
23   THRSERALAPHEGLYILEGLUPROASPTYR
24   GLYPHEALAILEASPVALTHRILEALAALA
25   ASPILEPROGLYTHRPROGLUHISLYSGLN
26   VALTHRHISLEUGLYLYSGLYTHRALAILE
27   LYSILEMETASPARGSERVALILECYSHIS
28   PROTHRILEVALARGTRPLEUGLUGLULEU
29   ALALYSLYSHISGLUILEPROTYRGLNLEU
30   GLUILELEULEUGLYGLYGLYTHRASPALA
31   GLYALAILEHISLEUTHRLYSALAGLYVAL
32   PROTHRGLYALALEUSERVALPROALAARG
33   TYRILEHISSERASNTHRGLUVALVALASP
34   GLUARGASPVALASPALATHRVALGLULEU
35   METTHRLYSALALEUGLUASNILEHISGLU
36   LEULYSILE

Samples:

sample_2: TRIS 50 ± 5 mM; sodium chloride 20 ± 1 mM; TET, [U-13C; U-15N; U-2H], 50 ± 10 % w/w

sample_ILV: TRIS 50 ± 5 mM; sodium chloride 20 ± 1 mM; TET, 13C,15N at Ile, Leu, Val, 50 ± 10 % w/w

sample_LKP: TRIS 50 ± 5 mM; sodium chloride 20 ± 1 mM; TET, 13C,15N at Leu, Lys, Pro, 50 ± 10 % w/w

sample_GYFR: TRIS 50 ± 5 mM; sodium chloride 20 ± 1 mM; TET, 13C,15N at Gly, Tyr, Phe, Arg, 50 ± 10 % w/w

sample_1: TRIS 50 ± 5 mM; sodium chloride 20 ± 1 mM; TET, [U-99% 13C; U-99% 15N], 50 ± 10 % w/w

sample_conditions_1: ionic strength: 0.07 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
CCdream (CC)sample_1isotropicsample_conditions_1
CANCO (hCANCO)sample_1isotropicsample_conditions_1
Expt_3 (hCA[N])sample_1isotropicsample_conditions_1
NCO (hNCO)sample_1isotropicsample_conditions_1
CC_ILV (CC)sample_ILVisotropicsample_conditions_1
ILV_NCA (hCA[N])sample_1isotropicsample_conditions_1
ILV_coNCA (hCA[N])sample_ILVisotropicsample_conditions_1
1D_13C (C)sample_1isotropicsample_conditions_1
HCC (H[C[C]])sample_1isotropicsample_conditions_1
NCA_on (hCA[N])sample_1isotropicsample_conditions_1
NCOCX (NCOCA)sample_1isotropicsample_conditions_1
ILV_NCO (hNCO)sample_ILVisotropicsample_conditions_1
CANcoCX (ca_NCO_C.onebond,relayed)sample_1isotropicsample_conditions_1
NCO_1000MHz (hNCO)sample_1isotropicsample_conditions_1
NCA_1000MHz (hCA[N])sample_1isotropicsample_conditions_1
NCACX_1000MHz (N_CA_C.onebond,relayed)sample_1isotropicsample_conditions_1
NCACB_Lp (N_CA_CB.onebond,relayed-alternate)sample_1isotropicsample_conditions_1
4D_CANCOCX (CA_NCO_C.onebond,relayed)sample_1isotropicsample_conditions_1
DARR_600MHz (C_C.through-space)sample_1isotropicsample_conditions_1
ILV_NCACX (NCO_C.relayed)sample_ILVisotropicsample_conditions_1
NCOCX_GHz (NCO_C.relayed)sample_1isotropicsample_conditions_1
ILV_NCOCX (NCO_C.relayed)sample_ILVisotropicsample_conditions_1
4D_CBsample_1isotropicsample_conditions_1
NCA_longCP (hCA[N])sample_1isotropicsample_conditions_1
NCO_longCP (CO[N])sample_1isotropicsample_conditions_1
NCACX_longCP (N_CA_C.onebond,relayed)sample_1isotropicsample_conditions_1
NCA_LKP (N_CA.onebond)sample_LKPisotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_2isotropicsample_conditions_1
3D hCANHsample_2isotropicsample_conditions_1
3D hCONHsample_2isotropicsample_conditions_1
3D hcaCBcaNHsample_2isotropicsample_conditions_1
3D hcoCAcoNHsample_2isotropicsample_conditions_1
coNCA_LKPsample_LKPisotropicsample_conditions_1
LKP_NCAshortsample_LKPisotropicsample_conditions_1
NCAsample_GYFRisotropicsample_conditions_1
coNCAsample_GYFRisotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.1, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian UnityPlus 600 MHz
  • Bruker Avance 1000 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks