BMRB Entry 27197

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Dm. Par3 PDZ1 domain
Published: 2018-02-15

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27197

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Dm. Par3 PDZ1 domain

Deposition date:

2017-08-02

Original release date:

2018-02-15

Authors:

Salomon, Paulin; Renschler, Fabian; Bruekner, Susanne; Wiesner, Silke

Citation:

Citation: Renschler, Fabian; Bruekner, Susanne; Salomon, Paulin; Mukherjee, Amrita; Kullmann, Lars; Schutz-Stoffregen, Mira; Henzler, Christine; Pawson, Tony; Krahn, Michael; Wiesner, Silke. "Structural basis for the interaction between the cell polarity proteins Par3 and Par6" Sci. Signal. 11, 9899-9899 (2018).
PubMed: 29440511

Assembly members:

Assembly members:
Par3, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Par3: GAMGDGEMLLIINEYGSPLG LTALPDKEHGGGLLVQHVEP GSRAERGRLRRDDRILEING IKLIGLTESQVQEQLRRALE SSELRVRVLRGDRN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	374
15N chemical shifts	85
1H chemical shifts	597

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Dm. Par3 PDZ1 domain	1

Entities:

Entity 1, Dm. Par3 PDZ1 domain 94 residues - Formula weight is not available

Residues 1-3 represent a cloning artefact

1

GLY

ALA

MET

GLY

ASP

GLY

GLU

MET

LEU

2

ILE

ASN

GLU

TYR

GLY

SER

PRO

LEU

GLY

3

LEU

THR

ALA

LEU

PRO

ASP

LYS

GLU

HIS

GLY

4

GLY

LEU

VAL

GLN

HIS

VAL

GLU

PRO

5

GLY

SER

ARG

ALA

GLU

ARG

GLY

ARG

LEU

ARG

6

ARG

ASP

ARG

ILE

LEU

GLU

ILE

ASN

GLY

7

ILE

LYS

LEU

ILE

GLY

LEU

THR

GLU

SER

GLN

8

VAL

GLN

GLU

GLN

LEU

ARG

ALA

LEU

GLU

9

SER

GLU

LEU

ARG

VAL

ARG

VAL

LEU

ARG

10

GLY

ASP

ARG

ASN

Samples:

sample_1: Par3 PDZ1 domain, [U-100% 13C; U-100% 15N], 1 mM; NaCl 150 mM; DTT 1 mM; sodium azide 0.03 % w/v; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker DMX 600 MHz
Bruker DMX 800 MHz