BMRB Entry 27183

Title:
1H, 15N, 13C backbone resonance assignment of the C-terminal domain of Enzyme I from Thermoanaerobacter Tengcongensis
Deposition date:
2017-07-18
Original release date:
2017-11-07
Authors:
Dotas, Rochelle; Venditti, Vincenzo
Citation:

Citation: Dotas, Rochelle; Venditti, Vincenzo. "1H, 15N, 13C backbone resonance assignment of the C-terminal domain of enzyme I from Thermoanaerobacter tengcongensis"  Biomol. NMR Assignments 12, 103-106 (2018).
PubMed: 29064000

Assembly members:

Assembly members:
EIC_from_from_Thermoanaerobacter_Tengcongensis, polymer, 314 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermoanaerobacter tengcongensis   Taxonomy ID: 119072   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermoanaerobacter tengcongensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts821
15N chemical shifts264
1H chemical shifts264

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EIC subunit 11
2EIC subunit 21

Entities:

Entity 1, EIC subunit 1 314 residues - Formula weight is not available

1   METALAGLUTHRPROASPGLYLYSLYSVAL
2   METLEUALAALAASNILEGLYTHRPROLYS
3   ASPVALALASERALALEUALAASNGLYALA
4   GLUGLYVALGLYLEUPHEARGTHRGLUPHE
5   LEUTYRMETASPARGASNSERLEUPROSER
6   GLUGLUGLUGLNPHEGLUALATYRLYSGLU
7   VALVALGLULYSMETGLYGLYARGPROVAL
8   THRILEARGTHRLEUASPILEGLYGLYASP
9   LYSGLULEUPROTYRLEUASPMETPROLYS
10   GLUMETASNPROPHELEUGLYTYRARGALA
11   ILEARGLEUCYSLEUASPARGPROASPILE
12   PHELYSTHRGLNLEUARGALAILELEUARG
13   ALASERALATYRGLYASNVALGLNILEMET
14   TYRPROMETILESERSERVALGLUGLUVAL
15   ARGLYSALAASNSERILELEUGLUGLUVAL
16   LYSALAGLULEUASPARGGLUGLYVALLYS
17   TYRASPLYSGLUILELYSVALGLYILEMET
18   VALGLUILEPROSERALAALAVALTHRALA
19   ASPILELEUALALYSGLUVALASPPHEPHE
20   SERILEGLYTHRASNASPLEUTHRGLNTYR
21   THRLEUALAVALASPARGMETASNGLUHIS
22   VALLYSGLUTYRTYRGLNPROPHEHISPRO
23   ALAILELEUARGLEUVALLYSMETVALILE
24   ASPALAALAHISLYSGLUGLYLYSPHEALA
25   ALAMETCYSGLYGLUMETALAGLYASPPRO
26   LEUALAALAVALILELEULEUGLYLEUGLY
27   LEUASPGLUPHESERMETSERALATHRSER
28   ILEPROGLUILELYSASNILEILEARGASN
29   VALGLUTYRGLULYSALALYSGLUILEALA
30   GLULYSALALEUASNMETSERGLUALAARG
31   GLUILEGLULYSMETMETLYSASPVALILE
32   LYSASPILEGLY

Samples:

sample_1: EIC from from Thermoanaerobacter Tengcongensis, [U-100% 13C; U-100% 15N], 0.5-1 mM; sodium chloride 100 mM; TRIS 20 mM; Magnesium Chloride 4 mM; EDTA 1 mM

sample_conditions_1: pH: 7.4; temperature: 343 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks