BMRB Entry 27138

Title:
HypF-N backbone assignment in non-toxic oligomer solution conditions
Deposition date:
2017-06-12
Original release date:
2018-06-21
Authors:
Patel, Jayneil; De Simone, Alfonso; Xu, Yingqi
Citation:

Citation: Patel, Jayneil; Xu, Yingqi; Capitini, Claudia; Chiti, Fabrizio; De Simone, Alfonso. "Backbone NMR assignments of HypF-N under conditions generating toxic and non-toxic oligomers"  Biomol. NMR Assign. 12, 273-277 (2018).
PubMed: 29786756

Assembly members:

Assembly members:
N_terminal_domain_of_HypF_(HypF-N), polymer, 92 residues, 10464.89 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE_Th

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts79
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HypF-N1

Entities:

Entity 1, HypF-N 92 residues - 10464.89 Da.

1   GLYSERALALYSASNTHRSERCYSGLYVAL
2   GLNLEUARGILEARGGLYLYSVALGLNGLY
3   VALGLYPHEARGPROPHEVALTRPGLNLEU
4   ALAGLNGLNLEUASNLEUHISGLYASPVAL
5   CYSASNASPGLYASPGLYVALGLUVALARG
6   LEUARGGLUASPPROGLUTHRPHELEUVAL
7   GLNLEUTYRGLNHISCYSPROPROLEUALA
8   ARGILEASPSERVALGLUARGGLUPROPHE
9   ILETRPSERGLNLEUPROTHRGLUPHETHR
10   ILEARG

Samples:

sample_1: D2O 10%; TFA 20 mM; sodium chloride 330 mM; N terminal domain of HypF (HypF-N), [U-100% 13C; U-100% 15N], 200 mM

sample_conditions_1: ionic strength: 330 mM; pH: 1.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis v2.4, W.F. Vranken, W Boucher, T.J. Stevens, R.H. Fogh, A Pajon, M. Llinas, E.L. Ulrich, J.L. Markley, J Ionides and E.D. Laue - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks