BMRB Entry 27120

Title:
Backbone chemical shifts of a circularized type III antifreeze protein from Macrozoarces americanus
Deposition date:
2017-05-31
Original release date:
2017-07-06
Authors:
Stevens, Corey; Langelaan, David; Allingham, John; Davies, Peter
Citation:

Citation: Stevens, Corey; Semrau, Joanna; Chiriac, Dragos; Litschko, Morgan; Campbell, Robert; Langelaan, David; Smith, Steven; Davies, Peter; Allingham, John. "Peptide backbone circularization enhances antifreeze protein thermostability."  Protein Sci. 26, 1932-1941 (2017).
PubMed: 28691252

Assembly members:

Assembly members:
circAFP, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: ocean pout   Taxonomy ID: 8199   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Macrozoarces americanus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts216
15N chemical shifts69
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1circAFP1

Entities:

Entity 1, circAFP 74 residues - Formula weight is not available

This is a circularized protein.

1   CYSTRPGLUGLYALAALAASNGLNALASER
2   VALVALALAASNGLNLEUILEPROILEASN
3   THRALALEUTHRLEUVALMETMETARGSER
4   GLUVALVALTHRPROVALGLYILEPROALA
5   GLUASPILEPROARGLEUVALSERMETGLN
6   VALASNARGALAVALPROLEUGLYTHRTHR
7   LEUMETPROASPMETVALLYSGLYTYRALA
8   ALAARGGLYLYS

Samples:

sample_1: circAFP, [U-100% 13C; U-100% 15N], 3 mM; TRIS 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr_Analysis v2.4, CCPN, Varian - chemical shift assignment, collection, data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks