BMRB Entry 27085

Title:
1H, 13C, 15N backbone NMR assignment of N-terminal domain of SaHPF
Deposition date:
2017-04-27
Original release date:
2017-10-05
Authors:
Usachev, Konstantin; Ayupov, Rustam; Validov, Shamil; Khusainov, Iskander; Yusupov, Marat
Citation:

Citation: Usachev, Konstantin; Ayupov, Rustam; Validov, Shamil; Khusainov, Iskander; Yusupov, Marat. "NMR assignments of the N-terminal domain of Staphylococcus aureus hibernation promoting factor (SaHPF)"  Biomol. NMR Assignments 12, 85-89 (2018).
PubMed: 28980143

Assembly members:

Assembly members:
SaHPF_NTD, polymer, 110 residues, 12913.7078 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGS21A

Data typeCount
13C chemical shifts435
15N chemical shifts102
1H chemical shifts501

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SaHPF NTD1

Entities:

Entity 1, SaHPF NTD 110 residues - 12913.7078 Da.

1   METILEARGPHEGLUILEHISGLYASPASN
2   LEUTHRILETHRASPALAILEARGASNTYR
3   ILEGLUGLULYSILEGLYLYSLEUGLUARG
4   TYRPHEASNASPVALPROASNALAVALALA
5   HISVALLYSVALLYSTHRTYRSERASNSER
6   ALATHRLYSILEGLUVALTHRILEPROLEU
7   LYSASNVALTHRLEUARGALAGLUGLUARG
8   ASNASPASPLEUTYRALAGLYILEASPLEU
9   ILEASNASNLYSLEUGLUARGGLNVALARG
10   LYSTYRLYSTHRARGILEASNARGLYSSER
11   ARGASPARGGLYASPGLNGLUVALPHEVAL

Samples:

NTD-SaHPF: SaHPF NTD, [U-100% 13C; U-100% 15N], 0.62 mM; NH4Cl 200.00 mM

condition1: ionic strength: 0.200 M; pH: 7.600; pressure: 1.000 atm; temperature: 308.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCNTD-SaHPFisotropiccondition1
2D 1H-13C HSQC/HMQCNTD-SaHPFisotropiccondition1
2D 1H-13C HSQC/HMQCNTD-SaHPFisotropiccondition1
3D HNCANTD-SaHPFisotropiccondition1
3D CBCA(CO)NHNTD-SaHPFisotropiccondition1
3D HNCACBNTD-SaHPFisotropiccondition1
3D HNCONTD-SaHPFisotropiccondition1
HN(CA)CO (H[N[ca[CO]]])NTD-SaHPFisotropiccondition1
CC(CO)NH (C_coNH.relayed)NTD-SaHPFisotropiccondition1
3D H(CCO)NHNTD-SaHPFisotropiccondition1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks