BMRB Entry 27060

Title:
1H assignments for DecP-11 in solution
Deposition date:
2017-03-30
Original release date:
2017-09-15
Authors:
Fadel, Valmir; Cabrera, Marcia
Citation:

Citation: Martins, Danubia Batista; Vieira, Maira Ramos; Fadel, Valmir; Santana, Viviane Aparecida Camargo; Guerra, Mirian Elisa Rodrigues; Lima, Marta Lopes; Tempone, Andre; Dos Santos Cabrera, Marcia Perez. "Membrane targeting peptides toward antileishmanial activity: Design, structural determination and mechanism of interaction"  Biochim. Biophys. Acta ., 30248-30249 (2017).
PubMed: 28780126

Assembly members:

Assembly members:
DecP-11, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DecP-11: SWLSLIRKLINSLLX

Data sets:
Data typeCount
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Decp-11, chain 11
2Decp-11, chain 21

Entities:

Entity 1, Decp-11, chain 1 15 residues - Formula weight is not available

1   SERTRPLEUSERLEUILEARGLYSLEUILE
2   ASNSERLEULEUNH2

Samples:

sample_1: DecP-11 2.1 mM; D2O 5%; TFE, [U-99% 2H], 30%; DSS 0 mM

sample_conditions_1: pH: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1,9,1,7, Keller - chemical shift assignment

CNS v1,3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

UNIO_10 v2.0.2, Herrmann, T - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Unity 600 MHz