BMRB Entry 27058

Name: Resonance assignment of MOAG-4 at pH 6.0
Published: 2017-03-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27058

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignment of MOAG-4 at pH 6.0

Deposition date:

2017-03-28

Original release date:

2017-03-30

Authors:

Yoshimura, Yuichi; Holmberg, Mats; Kukic, Predrag; Andersen, Camilla; Mata-Cabana, Alejandro; Falsone, Fabio; Vendruscolo, Michele; Nollen, Ellen; Mulder, Frans

Citation:

Citation: Yoshimura, Yuichi; Holmberg, Mats; Kukic, Predrag; Andersen, Camilla; Mata-Cabana, Alejandro; Falsone, S Fabio; Vendruscolo, Michele; Nollen, Ellen; Mulder, Frans. "MOAG-4 promotes the aggregation of alpha-synuclein by competing with self-protective electrostatic interactions." J. Biol. Chem. 292, 8269-8278 (2017).
PubMed: 28336532

Assembly members:

Assembly members:
MOAG-4, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MOAG-4: MTRGNQRDLAREKNQKKLAD QKKRQGASGQDGNAGLSMDA RMNRDADVMRIKQEKAAAKK EAEAAAAAANAKKVAKVDPL KM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	314
15N chemical shifts	79
1H chemical shifts	413

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MOAG-4	1

Entities:

Entity 1, MOAG-4 82 residues - Formula weight is not available

1

MET

THR

ARG

GLY

ASN

GLN

ARG

ASP

LEU

ALA

2

ARG

GLU

LYS

ASN

GLN

LYS

LEU

ALA

ASP

3

GLN

LYS

ARG

GLN

GLY

ALA

SER

GLY

GLN

4

ASP

GLY

ASN

ALA

GLY

LEU

SER

MET

ASP

ALA

5

ARG

MET

ASN

ARG

ASP

ALA

ASP

VAL

MET

ARG

6

ILE

LYS

GLN

GLU

LYS

ALA

LYS

7

GLU

ALA

GLU

ALA

ASN

8

ALA

LYS

VAL

ALA

LYS

VAL

ASP

PRO

LEU

9

LYS

MET

Samples:

sample_1: MOAG-4, [U-13C; U-15N], 0.4 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 6.0; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1
3D (HN)CO(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)N(COCO)NH	sample_1	isotropic	sample_conditions_1
3D H(CC)(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CC(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks