BMRB Entry 27047

Name: Hamster PrP 90-231
Published: 2017-04-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27047

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Hamster PrP 90-231

Deposition date:

2017-03-10

Original release date:

2017-04-26

Authors:

Hansen, D. Fleming; Rizk, Paul; Chong, P. Andrew; Forman-Kay, Julie

Citation:

Citation: Malevanets, Anatoly; Chong, P. Andrew; Hansen, D. Fleming; Rizk, Paul; Sun, Yulong; Lin, Hong; Muhandiram, Ranjith; Chakrabartty, Avi; Kay, Lewis; Forman-Kay, Julie; Wodak, Shoshana. "Interplay of buried histidine protonation and protein stability in prion misfolding" Sci. Rep. 7, 882-882 (2017).
PubMed: 28408762

Assembly members:

Assembly members:
Hamster_PrP_90-231, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Golden hamster Taxonomy ID: 10036 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesocricetus auratus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pProEX-Htb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hamster_PrP_90-231: GAMGGQGGGTHNQWNKPSKP KTNMKHMAGAAAAGAVVGGL GGYMLGSAMSRPMMHFGNDW EDRYYRENMNRYPNQVYYRP VDQYNNQNNFVHDCVNITIK QHTVTTTTKGENFTETDIKI MERVVEQMCTTQYQKESQAY YDGRRS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
15N chemical shifts	90
1H chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hamster PrP 90-231	1

Entities:

Entity 1, Hamster PrP 90-231 146 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

GLN

GLY

THR

2

HIS

ASN

GLN

TRP

ASN

LYS

PRO

SER

LYS

PRO

3

LYS

THR

ASN

MET

LYS

HIS

MET

ALA

GLY

ALA

4

ALA

GLY

ALA

VAL

GLY

LEU

5

GLY

TYR

MET

LEU

GLY

SER

ALA

MET

SER

6

ARG

PRO

MET

HIS

PHE

GLY

ASN

ASP

TRP

7

GLU

ASP

ARG

TYR

ARG

GLU

ASN

MET

ASN

8

ARG

TYR

PRO

ASN

GLN

VAL

TYR

ARG

PRO

9

VAL

ASP

GLN

TYR

ASN

GLN

ASN

PHE

10

VAL

HIS

ASP

CYS

VAL

ASN

ILE

THR

ILE

LYS

11

GLN

HIS

THR

VAL

THR

LYS

GLY

12

GLU

ASN

PHE

THR

GLU

THR

ASP

ILE

LYS

ILE

13

MET

GLU

ARG

VAL

GLU

GLN

MET

CYS

THR

14

THR

GLN

TYR

GLN

LYS

GLU

SER

GLN

ALA

TYR

15

TYR

ASP

GLY

ARG

SER

Samples:

sample_1: Syrian Hamster Prion Protein 90-231, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM

sample_2: Syrian Hamster Prion Protein 90-231, [U-99% 13C; U-99% 15N], 0.5 mM; sodium acetate 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7; pressure: 1 atm; temperature: 293 K

sample_conditions_2: ionic strength: 20 mM; pH: 5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks