BMRB Entry 27035

Title:
Backbone assignment of human Ube2T
Deposition date:
2017-02-14
Original release date:
2017-04-25
Authors:
Bortoluzzi, Alessio; Morreale, Francesca; Walden, Helen; Ciulli, Alessio
Citation:

Citation: Morreale, Francesca; Bortoluzzi, Alessio; Chaugule, Viduth; Arkinson, Connor; Walden, Helen; Ciulli, Alessio. "Allosteric targeting of the Fanconi anemia ubiquitin-conjugating enzyme Ube2T by fragment screening"  J. Med. Chem. 60, 4093-4098 (2017).
PubMed: 28437106

Assembly members:

Assembly members:
Ube2T_(1-154), polymer, 154 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET28

Data sets:
Data typeCount
13C chemical shifts399
15N chemical shifts126
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ube2T (1-154)1

Entities:

Entity 1, Ube2T (1-154) 154 residues - Formula weight is not available

1   METGLNARGALASERARGLEULYSARGGLU
2   LEUHISMETLEUALATHRGLUPROPROPRO
3   GLYILETHRCYSTRPGLNASPLYSASPGLN
4   METASPASPLEUARGALAGLNILELEUGLY
5   GLYALAASNTHRPROTYRGLULYSGLYVAL
6   PHELYSLEUGLUVALILEILEPROGLUARG
7   TYRPROPHEGLUPROPROGLNILEARGPHE
8   LEUTHRPROILETYRHISPROASNILEASP
9   SERALAGLYARGILECYSLEUASPVALLEU
10   LYSLEUPROPROLYSGLYALATRPARGPRO
11   SERLEUASNILEALATHRVALLEUTHRSER
12   ILEGLNLEULEUMETSERGLUPROASNPRO
13   ASPASPPROLEUMETALAASPILESERSER
14   GLUPHELYSTYRASNLYSPROALAPHELEU
15   LYSASNALAARGGLNTRPTHRGLULYSHIS
16   ALAARGGLNLYS

Samples:

sample_1: Ube2T (1-154), [U-100% 13C; U-100% 15N], 360 uM; potassium phosphate 50 mM; sodium chloride 85 mM; DTT 1 mM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, Bruker Biospin, CCPN - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker Avance III HD 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks