BMRB Entry 27015

Title:
Chemical shift assignments (HN,N,CA,CB) of reduced c-NmDsbD
Deposition date:
2017-01-26
Original release date:
2017-06-30
Authors:
Smith, Roxanne; Mohanty, Biswaranjan; Williams, Martin; Heras, Begona; Scanlon, Martin
Citation:

Citation: Smith, Roxanne; Mohanty, Biswaranjan; Williams, Martin; Scanlon, Martin; Heras, Begona. "H(N), N, C(alpha) and C(beta) assignments of the two periplasmic domains of Neisseria meningitidis DsbD"  Biomol. NMR Assign. 11, 181-186 (2017).
PubMed: 28589218

Assembly members:

Assembly members:
Reduced_c-NmDsbD, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Neisseria meningitidis   Taxonomy ID: 487   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCSG7

Data sets:
Data typeCount
13C chemical shifts255
15N chemical shifts124
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal periplasmic domain NmDsbD1

Entities:

Entity 1, C-terminal periplasmic domain NmDsbD 139 residues - Formula weight is not available

1   METHISHISHISHISHISHISSERSERGLY
2   VALASPLEUGLYTHRGLUASNLEUTYRPHE
3   GLNSERASNALAMETPHEALAASPTHRALA
4   ALALEULYSALAALAMETASPTHRALALEU
5   LYSGLUHISPROASPLYSPROVALVALLEU
6   ASPPHETYRALAASPTRPCYSILESERCYS
7   LYSGLUMETALAALATYRTHRLEUASNGLN
8   PROGLUVALHISGLNALAVALASPMETGLU
9   ARGPHEPHEGLNILEASPVALTHRALAASN
10   LYSPROGLUHISGLNALALEULEULYSGLU
11   TYRGLYLEUPHEGLYPROPROGLYVALPHE
12   VALVALARGSERASPGLYSERARGSERGLU
13   PROLEULEUGLYPHEVALLYSALAASPLYS
14   PHEILEGLUTRPTYRGLUGLNASNARG

Samples:

sample_1: Reduced c-NmDsbD, [U-98% 13C; U-98% 15N], 1 mM; D2O 10%; H2O 90%; sodium phosphate 10 mM; sodium chloride 50 mM; DTT 40 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D [15N,1H]-HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

UNIO-MATCH v2.0.2, Volk,J., Herrmann,T.,Wuthrich,K. - chemical shift assignment

CARA, Keller, K. and Wuthrich, K. - chemical shift calculation, data analysis, peak picking

Topspin v3.2, Bruker - Acquisition, processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks