BMRB Entry 27003

Name: Backbone assignment of human 4E-BP1 (fragment 44 to 87)
Published: 2017-08-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27003

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of human 4E-BP1 (fragment 44 to 87)

Deposition date:

2017-01-19

Original release date:

2017-08-02

Authors:

Leger-Abraham, Melissa; Boeszoermenyi, Andras; Wagner, Gerhard; Arthanari, Haribabu; Sekiyama, Naotaka

Citation:

Citation: Sekiyama, Naotaka; Boeszoermenyi, Andras; Arthanari, Haribabu; Wagner, Gerhard; Leger-Abraham, Melissa. "(1)H, (13)C, and (15)N backbone chemical shift assignments of 4E-BP144-87 and 4E-BP144-87 bound to eIF4E" Biomol. NMR Assign. 11, 187-191 (2017).
PubMed: 28589219

Assembly members:

Assembly members:
4EBP1_44to87_unbound, polymer, 47 residues, 5124.7813 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
4EBP1_44to87_unbound: GEFSTTPGGTRIIYDRKFLM ECRNSPVTKTPPRDLPTIPG VTSPSSD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	108
15N chemical shifts	37
1H chemical shifts	37

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	4EBP1 44 to 87 unbound	1

Entities:

Entity 1, 4EBP1 44 to 87 unbound 47 residues - 5124.7813 Da.

G, E, F are not part of 4EBP1 sequence, they come from cleaving the tag

1

GLY

GLU

PHE

SER

THR

PRO

GLY

THR

2

ARG

ILE

TYR

ASP

ARG

LYS

PHE

LEU

MET

3

GLU

CYS

ARG

ASN

SER

PRO

VAL

THR

LYS

THR

4

PRO

ARG

ASP

LEU

PRO

THR

ILE

PRO

GLY

5

VAL

THR

SER

PRO

SER

ASP

Samples:

sample_1: 4EBP1_44to87_unbound, [U-13C; U-15N], 0.25 mM; NaH2PO4/Na2HPO4 20 mM; NaCl 50 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.0, Wuthrich - Backbone assignment

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks