BMRB Entry 26997

Title:
Backbone assignment of human 4E-BP1 (fragment 44 to 87) bound to mouse eIF4E
Deposition date:
2017-01-18
Original release date:
2017-08-02
Authors:
Leger-Abraham, Melissa; Wagner, Gerhard; Boeszoermenyi, Andras; Arthanari, Haribabu; Sekiyama, Naotaka
Citation:

Citation: Sekiyama, Naotaka; Boeszoermenyi, Andras; Arthanari, Haribabu; Wagner, Gerhard; Leger-Abraham, Melissa. "(1)H, (13)C, and (15)N backbone chemical shift assignments of 4E-BP144-87 and 4E-BP144-87 bound to eIF4E"  Biomol. NMR Assign. 11, 187-191 (2017).
PubMed: 28589219

Assembly members:

Assembly members:
4E-BP1_44to87, polymer, 47 residues, 5124.7813 Da.
eIF4E, polymer, 219 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30

Data sets:
Data typeCount
13C chemical shifts72
15N chemical shifts36
1H chemical shifts36

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human 4E-BP1 (fragment 44 to 87)1
2eIF4E2

Entities:

Entity 1, human 4E-BP1 (fragment 44 to 87) 47 residues - 5124.7813 Da.

1   GLYGLUPHESERTHRTHRPROGLYGLYTHR
2   ARGILEILETYRASPARGLYSPHELEUMET
3   GLUCYSARGASNSERPROVALTHRLYSTHR
4   PROPROARGASPLEUPROTHRILEPROGLY
5   VALTHRSERPROSERSERASP

Entity 2, eIF4E 219 residues - Formula weight is not available

1   GLYSERMETALATHRVALGLUPROGLUTHR
2   THRPROTHRTHRASNPROPROPROALAGLU
3   GLUGLULYSTHRGLUSERASNGLNGLUVAL
4   ALAASNPROGLUHISTYRILELYSHISPRO
5   LEUGLNASNARGTRPALALEUTRPPHEPHE
6   LYSASNASPLYSSERLYSTHRTRPGLNALA
7   ASNLEUARGLEUILESERLYSPHEASPTHR
8   VALGLUASPPHETRPALALEUTYRASNHIS
9   ILEGLNLEUSERSERASNLEUMETPROGLY
10   CYSASPTYRSERLEUPHELYSASPGLYILE
11   GLUPROMETTRPGLUASPGLULYSASNLYS
12   ARGGLYGLYARGTRPLEUILETHRLEUASN
13   LYSGLNGLNARGARGSERASPLEUASPARG
14   PHETRPLEUGLUTHRLEULEUCYSLEUILE
15   GLYGLUSERPHEASPASPTYRSERASPASP
16   VALCYSGLYALAVALVALASNVALARGALA
17   LYSGLYASPLYSILEALAILETRPTHRTHR
18   GLUCYSGLUASNARGASPALAVALTHRHIS
19   ILEGLYARGVALTYRLYSGLUARGLEUGLY
20   LEUPROPROLYSILEVALILEGLYTYRGLN
21   SERHISALAASPTHRALATHRLYSSERGLY
22   SERTHRTHRLYSASNARGPHEVALVAL

Samples:

sample_1: 4E-BP1_44to87, [U-13C; U-15N], 0.25 mM; NaH2PO4/Na2HPO4 20 mM; NaCl 50 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.070 M; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1isotropicsample_conditions_1
HNCAsample_1isotropicsample_conditions_1
HSQCsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1
HNCOCAsample_1isotropicsample_conditions_1

Software:

XEASY v1.9.0, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks