BMRB Entry 26971

Title:
Chemical shifts of Lc-LTP2 in complex with DHPC
Deposition date:
2016-12-14
Original release date:
2017-04-26
Authors:
Mineev, Konstantin; Zakhar, Shenkarev; Alexander, Arseniev
Citation:

Citation: Shenkarev, Zakhar; Melnikova, Daria; Finkina, Ekaterina; Sukhanov, Stanislav; Boldyrev, Ivan; Gizatullina, Albina; Mineev, Konstantin; Arseniev, Alexander; Ovchinnikova, Tatiana. "Ligand Binding Properties of the Lentil Lipid Transfer Protein: Molecular Insight into the Possible Mechanism of Lipid Uptake"  Biochemistry 56, 1785-1796 (2017).
PubMed: 28266846

Assembly members:

Assembly members:
Ltp2, polymer, 93 residues, Formula weight is not available
1,2-dihexanoyl-sn-glycero-3-phosphocholine, non-polymer, 454.515 Da.

Natural source:

Natural source:   Common Name: lentil   Taxonomy ID: 3864   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: lens culinaris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21

Data sets:
Data typeCount
13C chemical shifts362
15N chemical shifts97
1H chemical shifts615

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ltp21
2DHPC2

Entities:

Entity 1, Ltp2 93 residues - Formula weight is not available

1   ALAILESERCYSGLYALAVALTHRSERASP
2   LEUSERPROCYSLEUTHRTYRLEUTHRGLY
3   GLYPROGLYPROSERPROGLNCYSCYSGLY
4   GLYVALLYSLYSLEULEUALAALAALAASN
5   THRTHRPROASPARGGLNALAALACYSASN
6   CYSLEULYSSERALAALAGLYSERILETHR
7   LYSLEUASNTHRASNASNALAALAALALEU
8   PROGLYLYSCYSGLYVALASNILEPROTYR
9   LYSILESERTHRTHRTHRASNCYSASNTHR
10   VALLYSPHE

Entity 2, DHPC - C20 H41 N O8 P - 454.515 Da.

1   HXG

Samples:

sample_1: Ltp2, [U-100% 13C; U-100% 15N], 1 mM; DMPC 10 mM; DHPC 15 mM

sample_conditions_1: ionic strength: 10 mM; pH: 5.7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CARA v1.9.4, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP A0AT29
AlphaFold P84255

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks