BMRB Entry 26965

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for an Exon-1 Fragment of Huntingtin at pH = 7.1 and T = 4 C
Deposition date:
2016-12-01
Original release date:
2016-12-29
Authors:
Baias, Maria; Smith, Pieter; Shen, Koning; Joachimiak, Lukasz; Zerko, Szymon; Kozminski, Wiktor; Frydman, Judith; Frydman, Lucio
Citation:

Citation: Baias, Maria; Smith, Pieter; Shen, Koning; Joachimiak, Lukasz; Zerko, Szymon; Kozminski, Wiktor; Frydman, Judith; Frydman, Lucio. "Structure and Dynamics of the Huntingtin Exon-1 N-Terminus: A Solution NMR Perspective"  J. Am. Chem. Soc. 139, 1168-1176 (2017).
PubMed: 28085263

Assembly members:

Assembly members:
Htt, polymer, 49 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJexpress411

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts96
15N chemical shifts32
1H chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Htt1

Entities:

Entity 1, Htt 49 residues - Formula weight is not available

1   METALATHRLEUGLULYSLEUMETLYSALA
2   PHEGLUSERLEULYSSERPHEGLNGLNGLN
3   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
4   GLNGLNGLNGLNPROPROPROPROPROPRO
5   LEUGLUCYSHISHISHISHISHISHIS

Samples:

sample_1: Htt, [U-13C; U-15N], 125 uM; D2O, [U-2H], 10%; DTT 5 mM; sodium phosphate 50 mM

sample_conditions_1: pH: 7.1; pressure: 1 atm; temperature: 277 K

sample_conditions_2: pressure: 1 atm; temperature: 277 K

sample_conditions_3: pH: 1.7; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D BEST 1H-15N HSQCsample_1isotropicsample_conditions_3
2D BEST 1H-15N HSQCsample_1isotropicsample_conditions_2
2D BEST 1H-15N HSQCsample_1isotropicsample_conditions_1
3D BEST HNCAsample_1isotropicsample_conditions_1
3D BEST HN(CO)CAsample_1isotropicsample_conditions_1
3D BEST HNCACBsample_1isotropicsample_conditions_1
3D BEST CBCA(CO)NHsample_1isotropicsample_conditions_1
3D BEST HN(CA)COsample_1isotropicsample_conditions_1
3D BEST HNCOsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks