BMRB Entry 26916

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26916

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Title:
Recombinant Human Granulocyte-Colony Stimulating Factor variant C3 (G-CSFC3) backbone assignments
Deposition date:
2016-10-08
Original release date:
2016-10-28
Authors:
Thomas, Rhys
Citation:

Citation: Thomas, Rhys; Kalverda, Arnout; Stewart, Katie; Buchanan, Andrew; Radford, Sheena; Brockwell, David. "Elucidating a role for protein dynamics in the aggregation of a Granulocyte-Colony Stimulating Factor variant"  .

Assembly members:

Assembly members:
G-CSFC3, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G-CSFC3: MTPLGPASSLPQSFLLKGLE QVRKIQGDGAALQEKLCATY KLCHPEELVLLGHSLGIPRA PLSSCPSQALRLAGCLSQLH SGLLLYQGLLQALEGISPEL GPTLDTLQLDVADFATTIWQ QMEELGMAPALQPTQGAMPA FASAFQRRAGGVLVASHLQS FLEVSYRVLRHLAQP

Data typeCount
13C chemical shifts909
15N chemical shifts324
1H chemical shifts324

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1G-CSFC31

Entities:

Entity 1, G-CSFC3 175 residues - Formula weight is not available

G-CSFC3 is identical to wild type human G-CSF (expressed recombinantly in E. coli) apart from substitutions C18G, W59R, Q71R and F84L.

1   METTHRPROLEUGLYPROALASERSERLEU
2   PROGLNSERPHELEULEULYSGLYLEUGLU
3   GLNVALARGLYSILEGLNGLYASPGLYALA
4   ALALEUGLNGLULYSLEUCYSALATHRTYR
5   LYSLEUCYSHISPROGLUGLULEUVALLEU
6   LEUGLYHISSERLEUGLYILEPROARGALA
7   PROLEUSERSERCYSPROSERGLNALALEU
8   ARGLEUALAGLYCYSLEUSERGLNLEUHIS
9   SERGLYLEULEULEUTYRGLNGLYLEULEU
10   GLNALALEUGLUGLYILESERPROGLULEU
11   GLYPROTHRLEUASPTHRLEUGLNLEUASP
12   VALALAASPPHEALATHRTHRILETRPGLN
13   GLNMETGLUGLULEUGLYMETALAPROALA
14   LEUGLNPROTHRGLNGLYALAMETPROALA
15   PHEALASERALAPHEGLNARGARGALAGLY
16   GLYVALLEUVALALASERHISLEUGLNSER
17   PHELEUGLUVALSERTYRARGVALLEUARG
18   HISLEUALAGLNPRO

Samples:

G-CSFC3_pH4: G-CSFC3, [U-13C; U-15N], 0.47 mM; sodium phosphate 18 mM; sodium acetate 18 mM; H2O 90%; D2O 10%

G-CSFC3_pH7: G-CSFC3, [U-13C; U-15N], 0.46 mM; sodium phosphate 18 mM; sodium acetate 18 mM; sodium azide 0.018 % w/v; H2O 90%; D2O 10%

G-CSFC3_pH4_conditions: ionic strength: 39 mM; pH: 4; pressure: 1 atm; temperature: 298 K

G-CSFC3_pH7_conditions: ionic strength: 68 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCG-CSFC3_pH4isotropicG-CSFC3_pH4_conditions
3D CBCA(CO)NHG-CSFC3_pH4isotropicG-CSFC3_pH4_conditions
3D HNCAG-CSFC3_pH4isotropicG-CSFC3_pH4_conditions
3D HN(CO)CAG-CSFC3_pH4isotropicG-CSFC3_pH4_conditions
3D HNCOG-CSFC3_pH4isotropicG-CSFC3_pH4_conditions
3D HN(CA)COG-CSFC3_pH4isotropicG-CSFC3_pH4_conditions
2D 1H-15N HSQCG-CSFC3_pH7isotropicG-CSFC3_pH7_conditions
3D CBCA(CO)NHG-CSFC3_pH7isotropicG-CSFC3_pH7_conditions
3D HNCAG-CSFC3_pH7isotropicG-CSFC3_pH7_conditions
3D HN(CO)CAG-CSFC3_pH7isotropicG-CSFC3_pH7_conditions
3D HNCOG-CSFC3_pH7isotropicG-CSFC3_pH7_conditions
3D HN(CA)COG-CSFC3_pH7isotropicG-CSFC3_pH7_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

VNMRJ, Varian - collection

CcpNmr_Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks