BMRB Entry 26907

Title:
Chemical shift assignments of a hydrophobin from Phanerochaete carnosa
Deposition date:
2016-09-23
Original release date:
2017-04-26
Authors:
Langelaan, David; Smith, Steven
Citation:

Citation: Gandier, Julie-Anne; Langelaan, David; Won, Amy; O'Donnell, Kylie; Grondin, Julie; Spencer, Holly; Wong, Philip; Tillier, Elisabeth; Yip, Christopher; Smith, Steven; Master, Emma. "Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision"  Sci. Rep. 7, 45863-45863 (2017).
PubMed: 28393921

Assembly members:

Assembly members:
hfb4, polymer, 126 residues, 12301.7867 Da.

Natural source:

Natural source:   Common Name: Phanerochaete carnosa   Taxonomy ID: 231932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Phanerochaete carnosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 32

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts79
1H chemical shifts475

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hfb41

Entities:

Entity 1, hfb4 126 residues - 12301.7867 Da.

1   LYSALAMETALAASPILEGLYSERTHRPRO
2   ASNPROGLUASNALALYSARGTRPALATHR
3   THRTHRTHRPROGLNALAPROTHRTHRTHR
4   VALTHRVALTHRGLUTHRALATHRALASER
5   SERVALPROALASERASPCYSSERTHRGLY
6   SERVALGLNCYSCYSASNTHRVALTHRSER
7   ALASERASPPROTHRALAALAGLYILELEU
8   GLYLEULEUGLYVALVALVALGLNGLYVAL
9   ASPVALLEUVALGLYLEUTHRCYSASPPRO
10   ILETHRVALVALGLYALAGLYGLYGLYSER
11   CYSSERALAGLNALAVALCYSCYSGLNASP
12   ASNSERTYRGLYSERLEUILESERILEGLY
13   CYSILEPROVALTHRLEU

Samples:

sample_1: PcaHyd1, [U-99% 13C; U-99% 15N], 1 mM; TRIS 25 mM; sodium chloride 150 mM; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking, processing

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks