BMRB Entry 26863

Title:
ScribPDZ4apo
Deposition date:
2016-07-30
Original release date:
2016-08-23
Authors:
Umitsu, Masataka; Ikura, Mitsuhiko
Citation:

Citation: Zheng, Weiyue; Umitsu, Masataka; Jagan, Ishaan; Tran, Charles; Ishiyama, Noboru; BeGora, Michael; Araki, Kiyomi; Ohashi, Pamela; Ikura, Mitsuhiko; Muthuswamy, Senthil. "An interaction between Scribble and the NADPH oxidase complex controls M1 macrophage polarization and function"  Nat. Cell. Biol. 18, 1244-1252 (2016).
PubMed: 27694890

Assembly members:

Assembly members:
ScribPDZ4apo, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts176
15N chemical shifts82
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ScribPDZ4apo1

Entities:

Entity 1, ScribPDZ4apo 102 residues - Formula weight is not available

1   GLYSERHISMETPROGLYLEUARGGLULEU
2   CYSILEGLNLYSALAPROGLYGLUARGLEU
3   GLYILESERILEARGGLYGLYALAARGGLY
4   HISALAGLYASNPROARGASPPROTHRASP
5   GLUGLYILEPHEILESERLYSVALSERPRO
6   THRGLYALAALAGLYARGASPGLYARGLEU
7   ARGVALGLYLEUARGLEULEUGLUVALASN
8   GLNGLNSERLEULEUGLYLEUTHRHISGLY
9   GLUALAVALGLNLEULEUARGSERVALGLY
10   ASPTHRLEUTHRVALLEUVALCYSASPGLY
11   PHEGLU

Samples:

sample_1_15N: ScribPDZ4apo, [U-100% 15N], 0.2 mM; NaCl 100 mM

sample_2_15N13C: ScribPDZ4apo, [U-100% 13C; U-100% 15N], 0.1 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1_15Nisotropicsample_conditions_1
2D 1H-15N HSQCsample_2_15N13Cisotropicsample_conditions_1
3D CBCA(CO)NHsample_2_15N13Cisotropicsample_conditions_1
3D HNCACBsample_2_15N13Cisotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks