BMRB Entry 26845

Title:
Field dependent R1 and R2 relaxation in B3 domain of protein G (GB3)
Deposition date:
2016-07-05
Original release date:
2016-12-29
Authors:
Hernandez, Griselda; LeMaster, David
Citation:

Citation: Hernandez, Griselda; LeMaster, David. "Quantifying protein dynamics in the ps-ns time regime by NMR relaxation"  J. Biomol. NMR 66, 163-174 (2016).
PubMed: 27734179

Assembly members:

Assembly members:
GB3, polymer, 56 residues, 6207.8 Da.

Natural source:

Natural source:   Common Name: Streptococcus   Taxonomy ID: 1301   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Data typeCount
T1 relaxation values204
T2 relaxation values204

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB31

Entities:

Entity 1, GB3 56 residues - 6207.8 Da.

1   METGLNTYRLYSLEUVALILEASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRLYSALA
3   VALASPALAGLUTHRALAGLULYSALAPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYVALTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

sample_1: GB3, [U-99% 15N], 2 mM; sodium phosphate 25 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 30 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1_relaxation_500sample_1isotropicsample_conditions_1
T1_relaxation_600sample_1isotropicsample_conditions_1
T1_relaxation_800sample_1isotropicsample_conditions_1
T1_relaxation_900sample_1isotropicsample_conditions_1
T2_relaxation_500sample_1isotropicsample_conditions_1
T2_relaxation_600sample_1isotropicsample_conditions_1
T2_relaxation_800sample_1isotropicsample_conditions_1
T2_relaxation_900sample_1isotropicsample_conditions_1

Software:

Felix, Felix NMR - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

GB AAA26921