BMRB Entry 26839

Title:
Resonance assignments of the charge assembly helix from the murine leukemia virus
Deposition date:
2016-06-25
Original release date:
2016-08-08
Authors:
Dolezal, Michal
Citation:

Citation: Dolezal, Michal; Hahravova, Romana; Kozisek, Milan; Bednarova, Lucie; Langerova, Hana; Ruml, Tomas; Rumlova, Michaela. "Functional and Structural Characterization of Novel Type of Linker Connecting Capsid and Nucleocapsid Protein Domains in Murine Leukemia Virus"  J. Biol. Chem. 291, 20630-20642 (2016).
PubMed: 27514744

Assembly members:

Assembly members:
MLV-CAH, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: murine leukemia virus   Taxonomy ID: 11786   Superkingdom: Viruses   Kingdom: not available   Genus/species: gammaretrovirus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts203
15N chemical shifts69
1H chemical shifts236

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MLV-CAH1

Entities:

Entity 1, MLV-CAH 72 residues - Formula weight is not available

residues 1-26 are an affinity tag

1   SERTYRTYRHISHISHISHISHISHISASP
2   TYRASPILEPROTHRTHRGLUASNLEUTYR
3   PHEGLNGLYALAMETALALYSARGGLUTHR
4   PROGLUGLUARGGLUGLUARGILEARGARG
5   GLUILEGLUGLULYSGLUGLUARGARGARG
6   ALAGLUASPGLUGLNARGGLUARGGLUARG
7   ASPARGARGARGHISARGGLUMETSERLYS
8   LEULEU

Samples:

sample_1: MLV-CAH, [U-99% 13C; U-99% 15N], 0.6 mM; sodium phosphate 50 mM; sodium chloride 50 mM; TCEP 1 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 0.350 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D CACOsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CONCAsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.3, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks