BMRB Entry 26803

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26803

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Title:
Recombinant human carcinoembryonic antigen related cell adhesion molecule 1 (hCEACAM1) N-terminal Igv domain
Deposition date:
2016-05-18
Original release date:
2016-08-31
Authors:
Prestegard, James; Zhuo, You
Citation:

Citation: Zhuo, You; Yang, Jeong-Yeh; Moremen, Kelley; Prestegard, James. "Glycosylation Alters Dimerization Properties of a Cell-surface Signaling Protein, Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1)"  J. Biol. Chem. 291, 20085-20095 (2016).
PubMed: 27471271

Assembly members:

Assembly members:
CEACAM1-Igv, polymer, 120 residues, 13371.78 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CEACAM1-Igv: MAQLTTESMPFNVAEGKEVL LLVHNLPQQLFGYSWYKGER VDGNRQIVGYAIGTQQATPG PANSGRETIYPNASLLIQNV TQNDTGFYTLQVIKSDLVNE EATGQFHVYAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CEACAM1-Igv, chain 11
2CEACAM1-Igv, chain 21

Entities:

Entity 1, CEACAM1-Igv, chain 1 120 residues - 13371.78 Da.

Residues 110-120 represent a non-native His-tag.

1   METALAGLNLEUTHRTHRGLUSERMETPRO
2   PHEASNVALALAGLUGLYLYSGLUVALLEU
3   LEULEUVALHISASNLEUPROGLNGLNLEU
4   PHEGLYTYRSERTRPTYRLYSGLYGLUARG
5   VALASPGLYASNARGGLNILEVALGLYTYR
6   ALAILEGLYTHRGLNGLNALATHRPROGLY
7   PROALAASNSERGLYARGGLUTHRILETYR
8   PROASNALASERLEULEUILEGLNASNVAL
9   THRGLNASNASPTHRGLYPHETYRTHRLEU
10   GLNVALILELYSSERASPLEUVALASNGLU
11   GLUALATHRGLYGLNPHEHISVALTYRALA
12   ALAALALEUGLUHISHISHISHISHISHIS

Samples:

sample_1: CEACAM1-Igv, [U-98% 13C; U-98% 15N], 200 uM; TRIS 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.007 M; pH: 7.4; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks