BMRB Entry 26754

Title:
BAZ2A PHD
Deposition date:
2016-03-09
Original release date:
2017-04-13
Authors:
Bortoluzzi, Alessio; Ciulli, Alessio
Citation:

Citation: Bortoluzzi, Alessio; Amato, Anastasia; Lucas, Xavier; Blank, Manuel; Ciulli, Alessio. "Structural Basis of Molecular Recognition of Helical Histone H3 Tail by PHD Finger Domains"  Biochem J. 474, 1633-1651 (2017).
PubMed: 28341809

Assembly members:

Assembly members:
BAZ2A_PHD, polymer, 58 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Modified pET15

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts160
15N chemical shifts53
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BAZ2A PHD1
2Zinc12
3Zinc22

Entities:

Entity 1, BAZ2A PHD 58 residues - Formula weight is not available

1   HISMETSERVALASNLYSVALTHRCYSLEU
2   VALCYSARGLYSGLYASPASNASPGLUPHE
3   LEULEULEUCYSASPGLYCYSASPARGGLY
4   CYSHISILETYRCYSHISARGPROLYSMET
5   GLUALAVALPROGLUGLYASPTRPPHECYS
6   THRVALCYSLEUALAGLNGLNVAL

Entity 2, Zinc1 - 65.409 Da.

1   ZN

Samples:

sample_1: BAZ2A PHD, [U-98% 13C; U-98% 15N], 350 uM; DTT 1 mM; potassium chloride 50 mM; potassium phosphate 25 mM; sodium azide 0.02 w/v; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.200 M; pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CcpNmr_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III HD 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks