BMRB Entry 26727

Title:
1H, 13C and 15N assignment for the C-terminal domain of human Doublecortin
Deposition date:
2016-01-10
Original release date:
2016-07-14
Authors:
Di Lello, Paola
Citation:

Citation: Burger, Dominique; Stihle, Martine; Sharma, Ashwani; Di Lello, Paola; Benz, Jorg; D'Arcy, Brigitte; Debulpaep, Maja; Fry, David; Huber, Walter; Kremer, Thomas; Laeremans, Toon; Matile, Hugues; Ross, Alfred; Rufer, Arne; Schoch, Guillaume; Steinmetz, Michel; Steyaert, Jan; Rudolph, Markus; Thoma, Ralf; Ruf, Armin. "Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies"  J. Biol. Chem. 291, 16292-16306 (2016).
PubMed: 27226599

Assembly members:

Assembly members:
Doublecortin_C-terminal_domain, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pER2b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts108
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Doublecortin C-terminal domain monomer1

Entities:

Entity 1, Doublecortin C-terminal domain monomer 114 residues - Formula weight is not available

1   LEUVALPROARGGLYSERHISMETALAARG
2   GLUASNLYSASPPHEVALARGPROLYSLEU
3   VALTHRILEILEARGSERGLYVALLYSPRO
4   ARGLYSALAVALARGVALLEULEUASNLYS
5   LYSTHRALAHISSERPHEGLUGLNVALLEU
6   THRASPILETHRGLUALAILELYSLEUGLU
7   THRGLYVALVALLYSLYSLEUTYRTHRLEU
8   ASPGLYLYSGLNVALTHRCYSLEUHISASP
9   PHEPHEGLYASPASPASPVALPHEILEALA
10   CYSGLYPROGLULYSPHEARGTYRALAGLN
11   ASPASPPHESERLEUASPGLUASNGLUCYS
12   ARGVALMETLYS

Samples:

sample_1: Doublecortin C-terminal domain, [U-99% 13C; U-99% 15N], 0.5 mM; sodium acetate 20 mM; TCEP 5 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 20 mM; pH: 4.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (HB)CBCA(CO)NNHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks